Muratova Anna, Golubev Sergey, Romanova Valeria, Sungurtseva Irina, Nurzhanova Asil
Institute of Biochemistry and Physiology of Plants and Microorganisms, Saratov Scientific Centre of the Russian Academy of Sciences (IBPPM RAS), 410049 Saratov, Russia.
Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, 420021 Kazan, Russia.
Microorganisms. 2023 Jun 7;11(6):1516. doi: 10.3390/microorganisms11061516.
Microbial-assisted phytoremediation is considered a more effective approach to soil rehabilitation than the sole use of plants. sp. Pb113 and sp. Zn19, heavy-metal-resistant PGPR strains originally isolated from the rhizosphere of , were used as inoculants of the host plant grown in control and zinc-contaminated (1650 mg/kg) soil in a 4-month pot experiment. The diversity and taxonomic structure of the rhizosphere microbiomes, assessed with metagenomic analysis of rhizosphere samples for the 16S rRNA gene, were studied. Principal coordinate analysis showed differences in the formation of the microbiomes, which was affected by zinc rather than by the inoculants. Bacterial taxa affected by zinc and the inoculants, and the taxa potentially involved in the promotion of plant growth as well as in assisted phytoremediation, were identified. Both inoculants promoted miscanthus growth, but only sp. Zn19 contributed to significant Zn accumulation in the aboveground part of the plant. In this study, the positive effect of miscanthus inoculation with spp. and spp. was demonstrated for the first time. On the basis of our data, the bacterial strains studied may be recommended to improve the efficiency of phytoremediation of zinc-contaminated soil.
微生物辅助植物修复被认为是一种比单纯使用植物更有效的土壤修复方法。最初从多花黑麦草根际分离出的重金属抗性植物根际促生菌（PGPR）菌株铅113和锌19，在一项为期4个月的盆栽试验中，被用作在对照土壤和锌污染土壤（1650毫克/千克）中生长的宿主植物的接种剂。通过对根际样品的16S rRNA基因进行宏基因组分析，研究了根际微生物群落的多样性和分类结构。主坐标分析表明微生物群落的形成存在差异，这种差异受锌的影响而非接种剂的影响。确定了受锌和接种剂影响的细菌分类群，以及可能参与促进植物生长和辅助植物修复的分类群。两种接种剂都促进了芒草的生长，但只有锌19菌株促使植物地上部分显著积累锌。在本研究中，首次证明了用铅113菌株和锌19菌株接种芒草具有积极效果。根据我们的数据，所研究的细菌菌株可能有助于提高锌污染土壤的植物修复效率。
