Kausar J, Ohyama Y, Yamamoto O
Division of Gene Chemistry, Graduate School of Gene Science, Faculty of Sciences, Hiroshima University, Higashi-Hiroshima, Japan.
Biochem Mol Biol Int. 1996 Jul;39(4):697-702. doi: 10.1080/15216549600201771.
Partial sequence of the 16S ribosomal RNA gene of an extremely high radiotolerant bacterium, Rubrobacter radiotolerans (reclassified from Arthrobacter radiotolerans by chemical characteristics) was determined by PCR-amplification from a small amount of heat-lysed biomass, followed by direct sequencing of the PCR product. The sequence was aligned with seven species of Arthrobacter and also with representatives of various other bacterial groups. R. radiotolerans was confirmed to be out of the Arthrobacter group justifying the reclassification. Moreover, it has an individual position among the selected representatives being closer to the eubacterial groups.
通过对少量经热裂解的生物量进行PCR扩增，随后对PCR产物进行直接测序，确定了一种极端耐辐射细菌——耐辐射红球菌（根据化学特征从耐辐射节杆菌重新分类而来）的16S核糖体RNA基因的部分序列。该序列与七种节杆菌属物种以及其他各种细菌类群的代表序列进行了比对。耐辐射红球菌被证实不属于节杆菌属，这证明了重新分类的合理性。此外，在所选的代表物种中，它具有独特的位置，更接近真细菌类群。
