Kausar J, Ohyama Y, Terato H, Ide H, Yamamoto O
Graduate Department of Gene Science, Faculty of Science, Hiroshima University, Japan.
Int J Syst Bacteriol. 1997 Jul;47(3):684-6. doi: 10.1099/00207713-47-3-684.
The nearly complete sequence of the 16S rRNA gene of an extremely highly radiotolerant bacterium, Rubrobacter radiotolerans (reclassified from Arthrobacter radiotolerans based on chemical characteristics), was determined by PCR amplification of the genomic DNA followed by cloning of the amplified gene and sequencing by the dideoxynucleotide method. The sequence was aligned with the sequences of members of the genus Arthrobacter and also with the sequences of representatives of the gram-positive bacteria having high G + C contents and the family Deinococcaceae (radioresistant micrococci and their relatives). The results of our phylogenetic analysis confirmed that R. radiotolerans is not a member of the Arthrobacter group and thus supported the previous reclassification. Moreover, although it is radioresistant and has a high G+C content, R. radiotolerans is more closely related to the gram-positive bacteria with high G+C contents than to the radioresistant members of the Deinococcaceae.
一株极端耐辐射细菌——耐辐射红球菌（基于化学特性从耐辐射节杆菌重新分类而来）的16S rRNA基因的近乎完整序列，通过对基因组DNA进行PCR扩增，随后克隆扩增基因并采用双脱氧核苷酸法进行测序得以确定。该序列与节杆菌属成员的序列以及高G + C含量的革兰氏阳性菌和嗜放射菌科（耐辐射微球菌及其亲缘种）代表的序列进行了比对。我们的系统发育分析结果证实，耐辐射红球菌不是节杆菌属的成员，从而支持了先前的重新分类。此外，尽管耐辐射红球菌具有抗辐射性且G + C含量高，但它与高G + C含量的革兰氏阳性菌的亲缘关系比与嗜放射菌科的抗辐射成员更近。
