Costa Monique Melo, Corbel Vincent, Ben Hamouda Refka, Almeras Lionel
Unité de Parasitologie et Entomologie, Département de Microbiologie et Maladies Infectieuses, Institut de Recherche Biomédicale des Armées, 13005 Marseille, France.
Aix Marseille Univ, SSA, AP-HM, RITMES, 13005 Marseille, France.
Insects. 2024 Aug 29;15(9):651. doi: 10.3390/insects15090651.
Mosquito-borne diseases are responsible for hundreds of thousands of deaths per year. The identification and control of the vectors that transmit pathogens to humans are crucial for disease prevention and management. Currently, morphological classification and molecular analyses via DNA barcoding are the standard methods used for vector identification. However, these approaches have several limitations. In the last decade, matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) profiling has emerged as an innovative technology in biological sciences and is now considered as a relevant tool for the identification of pathogens and arthropods. Beyond species identification, this tool is also valuable for determining various life traits of arthropod vectors. The purpose of the present systematic review was to highlight the contribution of MALDI-TOF MS to the surveillance and control of mosquito-borne diseases. Published articles from January 2003 to August 2024 were retrieved, focusing on different aspects of mosquito life traits that could be determinants in disease transmission and vector management. The screening of the scientific literature resulted in the selection of 54 published articles that assessed MALDI-TOF MS profiling to study various mosquito biological factors, such species identification, life expectancy, gender, trophic preferences, microbiota, and insecticide resistance. Although a large majority of the selected articles focused on species identification, the present review shows that MALDI-TOF MS profiling is promising for rapidly identifying various mosquito life traits, with high-throughput capacity, reliability, and low cost. The strengths and weaknesses of this proteomic tool for vector control and surveillance are discussed.
蚊媒疾病每年导致数十万人死亡。识别并控制将病原体传播给人类的病媒对于疾病预防和管理至关重要。目前，形态学分类和通过DNA条形码进行的分子分析是用于病媒识别的标准方法。然而，这些方法存在若干局限性。在过去十年中，基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）分析已成为生物科学中的一项创新技术，现在被认为是识别病原体和节肢动物的相关工具。除了物种识别外，该工具对于确定节肢动物病媒的各种生活特征也很有价值。本系统综述的目的是强调MALDI-TOF MS对蚊媒疾病监测和控制的贡献。检索了2003年1月至2024年8月发表的文章，重点关注可能是疾病传播和病媒管理决定因素的蚊子生活特征的不同方面。对科学文献的筛选导致选择了54篇已发表的文章，这些文章评估了MALDI-TOF MS分析以研究各种蚊子生物学因素，如物种识别、预期寿命、性别、营养偏好、微生物群和杀虫剂抗性。尽管绝大多数所选文章侧重于物种识别，但本综述表明，MALDI-TOF MS分析在快速识别各种蚊子生活特征方面具有前景，具有高通量能力、可靠性和低成本。讨论了这种蛋白质组学工具在病媒控制和监测方面的优缺点。
