Liu Q, Hobom G
Institute of Microbiology and Molecular Biology, University of Giessen, Germany.
Virus Genes. 1999;19(3):183-7. doi: 10.1023/a:1008180529157.
Budgerigar fledgling disease virus (BFDV) genome contains two times two (two pairs) open reading frames (agnogenes) at the 5' end of the late coding region. Recombinant influenza A viruses were constructed to express the second pair of BFDV agnoproteins, agno-2a and agno-2b, with a fusion of a histidine-tag at their carboxy-termini, respectively. Specific proteins were detected in Western blot analysis using anti histidine-tag monoclonal antibody. By indirect immunofluorescence experiments agno-2a and agno-2b were shown to be located on the surface and in the perinuclear and cytoplasmic areas of infected cells. Comparisons of the expression patterns of BFDV agno-2a and agno-2b with that of simian virus 40 agnoprotein reveal high similarity, suggesting that they might have the same function(s) in polyomavirus infectious cycle.
虎皮鹦鹉幼雏病病毒（BFDV）基因组在晚期编码区的5'端包含两对开放阅读框（agnogenes）。构建了重组甲型流感病毒，以分别在其羧基末端融合组氨酸标签的方式表达第二对BFDV agnoprotein，即agno-2a和agno-2b。使用抗组氨酸标签单克隆抗体在蛋白质免疫印迹分析中检测到了特定蛋白质。通过间接免疫荧光实验表明，agno-2a和agno-2b位于感染细胞的表面以及核周和细胞质区域。将BFDV agno-2a和agno-2b的表达模式与猿猴病毒40 agnoprotein的表达模式进行比较，发现高度相似，这表明它们在多瘤病毒感染周期中可能具有相同的功能。
