Sigler K, Knotková A, Kotyk A
Folia Microbiol (Praha). 1978;23(6):409-22. doi: 10.1007/BF02885568.
Glucose-induced acid extrusion, respiration and anaerobic fermentation in baker's yeast was studied with the aid of sixteen inhibitors. Uranyl(2+) nitrate affected the acid extrusion more anaerobically than aerobically; the complexing of Mg2+ and Ca2+ by EDTA at the membrane had no effect. Inhibitors of glycolysis (iodoacetamide, N-ethylmaleimide, fluoride) suppressed acid production markedly, and so did the phosphorylation-blocking arsenate. Fluoroacetate, inhibiting the citric-acid cycle, had no effect. Inhibition by uncouplers depended on their pKa values: 2,4,6-trinitrophenol (pKa 0.4) less than 2,4-dinitrophenol (4.1) less than azide (4.7) less than 3-chlorophenylhydrazonomalononitrile (6.0). Inhibition by trinitrophenol was only slightly increased by its acetylation. Cyanide and nonpermeant oligomycin showed practically no effect; inhibition by dicyclohexylcarbodiimide was delayed but potent. The concentration profiles of inhibition of acid production differed from those of respiration and fermentation. Thus, though the acid production is a metabolically dependent process, it does not reflect the intensity of metabolism, except partly in the first half of glycolysis.
借助16种抑制剂，研究了葡萄糖诱导的面包酵母中的酸排出、呼吸作用和无氧发酵。硝酸铀酰（2+）在无氧条件下比在有氧条件下对酸排出的影响更大；膜上的EDTA对Mg2+和Ca2+的络合没有影响。糖酵解抑制剂（碘乙酰胺、N-乙基马来酰亚胺、氟化物）显著抑制酸的产生，磷酸化阻断剂砷酸盐也有同样的作用。抑制柠檬酸循环的氟乙酸没有效果。解偶联剂的抑制作用取决于它们的pKa值：2,4,6-三硝基苯酚（pKa 0.4）＜2,4-二硝基苯酚（4.1）＜叠氮化物（4.7）＜3-氯苯基腙基丙二腈（6.0）。三硝基苯酚的乙酰化仅使其抑制作用略有增加。氰化物和非渗透性寡霉素几乎没有作用；二环己基碳二亚胺的抑制作用延迟但很强。酸产生的抑制浓度曲线与呼吸作用和发酵的不同。因此，虽然酸的产生是一个代谢依赖的过程，但它并不反映代谢的强度，除了在糖酵解的前半部分有部分反映。
