Okorokov L A, Lichko L P, Kadomtseva V M, Kholodenko V P, Titovsky V T, Kulaev I S
Eur J Biochem. 1977 May 16;75(2):373-7. doi: 10.1111/j.1432-1033.1977.tb11538.x.
Manganese transport into yeast cells is energy-dependent. It is dependent on endogenous sources of energy and is inhibited by olygomycin (12.5-25 microgramg/ml), 2,4-dinitrophenol (1 mM), 2-deoxyglucose (1-50 mM) and sodium azide (1-10 mM), but is stimulated by cyanide and glucose. The stimulating effect of glucose is eliminated by N-ethylmaleimide and iodoacetate, which apparently inhibit the transport of glucose itself. About 75% of the manganese accumulated in the presence of glucose is found in yeast protoplasts and nearly 25% in the cell walls. A major portion of the accumulated manganese is found in vacuoles. The concentration of osmotically free manganese in the cytosol did not exceed 2 mM, but the concentration in vacuoles was up to 14 mM. The tonoplast is assumed to have a transport system for divalent cations, thereby regulating their concentration in the cytosol.
锰进入酵母细胞的过程是能量依赖型的。它依赖内源性能量来源，受到寡霉素（12.5 - 25微克/毫升）、2,4 - 二硝基苯酚（1毫摩尔）、2 - 脱氧葡萄糖（1 - 50毫摩尔）和叠氮化钠（1 - 10毫摩尔）的抑制，但受到氰化物和葡萄糖的刺激。葡萄糖的刺激作用被N - 乙基马来酰亚胺和碘乙酸消除，这两种物质显然抑制了葡萄糖自身的转运。在有葡萄糖存在的情况下积累的锰，约75%存在于酵母原生质体中，近25%存在于细胞壁中。积累的锰大部分存在于液泡中。胞质溶胶中渗透压自由锰的浓度不超过2毫摩尔，但液泡中的浓度高达14毫摩尔。液泡膜被认为具有二价阳离子转运系统，从而调节它们在胞质溶胶中的浓度。
