Meade C J, Sheena J, Mertin J
Int Arch Allergy Appl Immunol. 1979;58(2):121-7. doi: 10.1159/000232183.
Spleen cells from mice homozygous for the obese (ob) mutation killed DBA/2 mastocytoma target cells less well than spleen cells from lean littermates or unrelated age-and sex-matched controls of the same strain. Killing was impaired only when the attacker cells were primed in vivo, not following in vitro priming. Hence the effect of the ob/ob genotype is not to produce an irreversible functional change in the lymphocyte, but rather to produce an environment in which lymphocytes are less able to react to priming antigen. Not only were the spleen cells of in vivo primed obese mice less active than those of lean controls, but also their number per spleen was significantly decreased. Such a quantitive difference was no longer found in adrenalectomised animals, but the qualitative difference in spleen cell cytotoxic activity still occurred. This suggests that adrenocortical hyperfunction may affect immune function in obese mice, without necessarily being the only factor in the in vivo environment of obese mouse spleen cells capable of depressing cellular immune reactivity.
肥胖（ob）突变纯合子小鼠的脾细胞对DBA/2肥大细胞瘤靶细胞的杀伤能力，不如来自瘦的同窝小鼠或相同品系中年龄和性别匹配的无关对照的脾细胞。只有当攻击细胞在体内被致敏时，杀伤能力才会受损，体外致敏则不会。因此，ob/ob基因型的作用不是在淋巴细胞中产生不可逆的功能变化，而是产生一种淋巴细胞对致敏抗原反应能力较低的环境。体内致敏的肥胖小鼠的脾细胞不仅比瘦对照的脾细胞活性低，而且每个脾脏中的脾细胞数量也显著减少。在肾上腺切除的动物中不再发现这种数量差异，但脾细胞细胞毒性活性的质量差异仍然存在。这表明肾上腺皮质功能亢进可能会影响肥胖小鼠的免疫功能，但不一定是肥胖小鼠脾细胞体内环境中能够抑制细胞免疫反应性的唯一因素。
