由拟茎点霉属FT-0211菌株产生的吩恶嗪类化合物，是小鼠巨噬细胞中脂滴形成的抑制剂。

Phenochalasins, inhibitors of lipid droplet formation in mouse macrophages, produced by Phomopsis sp. FT-0211.

作者信息

Tomoda H, Namatame I, Si S, Kawaguchi K, Masuma R, Namikoshi M, Omura S

机构信息

Graduate School of Pharmaceutical Sciences, Kitasato University, Japan.

出版信息

J Antibiot (Tokyo). 1999 Oct;52(10):851-6. doi: 10.7164/antibiotics.52.851.

DOI:10.7164/antibiotics.52.851

PMID:10604753

Abstract

Phomopsis sp. FT-0211, a soil isolate, was found to produce inhibitors of lipid droplet formation in mouse peritoneal macrophages. Structurally related new compounds designated phenochalasins A and B were isolated from the fermentation broth of the producing strain by solvent extraction, ODS column chromatography and preparative HPLC. Phenochalasin A caused a dose-dependent reduction in the number and size of lipid droplets in macrophages without any cytotoxic effect at least up to 20 microm. On the other hand, phenochalasin B showed inhibition of lipid droplet formation with a severe cytotoxic effect on macrophages.

摘要

拟茎点霉属真菌FT-0211是一种从土壤中分离得到的菌株，被发现能够产生抑制小鼠腹腔巨噬细胞中脂滴形成的物质。通过溶剂萃取、ODS柱色谱法和制备型高效液相色谱法，从该产生菌的发酵液中分离出了结构相关的新化合物，命名为苯并查耳酮A和B。苯并查耳酮A能使巨噬细胞中脂滴的数量和大小呈剂量依赖性减少，至少在浓度高达20微摩尔时没有任何细胞毒性作用。另一方面，苯并查耳酮B显示出对脂滴形成的抑制作用，同时对巨噬细胞具有严重的细胞毒性作用。