二醇霉素，由链霉菌属产生的新型抗球虫剂。I. 生产、分离及物理化学和生物学特性。

Diolmycins, new anticoccidial agents produced by Streptomyces sp. I. Production, isolation and physico-chemical and biological properties.

作者信息

Tabata N, Tomoda H, Takahashi Y, Haneda K, Iwai Y, Woodruff H B, Omura S

机构信息

Research Center for Biological Function, Kitasato Institute, Tokyo, Japan.

出版信息

J Antibiot (Tokyo). 1993 May;46(5):756-61. doi: 10.7164/antibiotics.46.756.

DOI:10.7164/antibiotics.46.756

PMID:8514630

Abstract

Streptomyces sp. WK-2955, a soil isolate, was found to produce a series of new anticoccidial compounds. Four active compounds, designated diolmycins A1, A2, B1 and B2, were isolated from the fermentation broth of the producing strain by solvent extraction, silica gel column chromatography, gel filtration on Sephadex LH-20, and preparative HPLC. Diolmycins inhibited the growth of Eimeria tenella in an in vitro assay system using BHK-21 cells as a host. No schizont in the cells was observed at concentrations of 0.02-2.0 micrograms/ml for diolmycin A1, at 0.2-2.0 micrograms/ml for diolmycin A2, and at 20 micrograms/ml for diolmycins B1 and B2.

摘要

链霉菌属菌株WK - 2955是从土壤中分离得到的，被发现能产生一系列新的抗球虫化合物。通过溶剂萃取、硅胶柱色谱、Sephadex LH - 20凝胶过滤和制备型高效液相色谱，从该产生菌的发酵液中分离出四种活性化合物，分别命名为二醇霉素A1、A2、B1和B2。在以BHK - 21细胞为宿主的体外检测系统中，二醇霉素能抑制柔嫩艾美耳球虫的生长。对于二醇霉素A1，在浓度为0.02 - 2.0微克/毫升时细胞中未观察到裂殖体；二醇霉素A2在0.2 - 2.0微克/毫升时未观察到；二醇霉素B1和B2在20微克/毫升时未观察到。