Strickler H D, Goedert J J, Fleming M, Travis W D, Williams A E, Rabkin C S, Daniel R W, Shah K V
Viral Epidemiology Branch, National Cancer Institute, NIH, Bethesda, Maryland 20892, USA.
Cancer Epidemiol Biomarkers Prev. 1996 Jun;5(6):473-5.
It has been reported that DNA of SV40, a virus of Asian macaques that is tumorigenic for rodents and can transform human cells in vitro, is present in pleural mesotheliomas and in several other cancers. To verify these observations, we tested paraffin sections from mesothelioma tissues of 50 patients for SV40 DNA using PCR with two separate sets of primers. The analytic sensitivity for detection of SV40 DNA was 1-10 genome copies. We also tested the specimens for beta-globin by PCR to assess the suitability of the specimen DNAs for amplification. beta-Globin amplification was detected in 48 of the 50 specimens, but SV40 DNA was not detected in any tumors, with either of two SV40 primer sets. Furthermore, sera from 34 additional patients with mesothelioma, 33 patients with osteosarcoma (another cancer reported to be SV40-related) and 35 controls were tested for SV40 antibodies by a plaque neutralization assay. The serological data, like the DNA results, did not support an association of SV40 with mesothelioma or with osteosarcoma; antibodies to SV40 were detected in three mesothelioma patients, in one osteosarcoma patient, and in one control. These findings call into question the association of SV40 with mesothelioma.
据报道，猴空泡病毒40（SV40）是一种亚洲猕猴病毒，对啮齿动物具有致瘤性，且能在体外转化人类细胞，其DNA存在于胸膜间皮瘤和其他几种癌症中。为了验证这些观察结果，我们使用两组不同的引物通过聚合酶链反应（PCR）检测了50例患者间皮瘤组织石蜡切片中的SV40 DNA。检测SV40 DNA的分析灵敏度为1 - 10个基因组拷贝。我们还通过PCR检测了样本中的β - 珠蛋白，以评估样本DNA用于扩增的适用性。在50个样本中的48个检测到了β - 珠蛋白扩增，但使用两组SV40引物中的任何一组，在任何肿瘤中均未检测到SV40 DNA。此外，通过空斑中和试验检测了另外34例间皮瘤患者、33例骨肉瘤患者（另一种据报道与SV40相关的癌症）的血清以及35名对照者的血清中的SV40抗体。血清学数据与DNA检测结果一样，不支持SV40与间皮瘤或骨肉瘤有关联；在3例间皮瘤患者、1例骨肉瘤患者和1名对照者中检测到了抗SV40抗体。这些发现对SV40与间皮瘤的关联提出了质疑。
