David J P, Rey D, Pautou M P, Meyran J C
UMR Ecosystèmes et Changements Environnementaux, Université Joseph Fourier, Grenoble Cedex 9, F-38041, France.
J Invertebr Pathol. 2000 Jan;75(1):9-18. doi: 10.1006/jipa.1999.4886.
The relative toxicity of leaf litter to nematocerous dipteran larvae characteristic of mosquito developmental sites was investigated. Culicidae, Chironomidae, and Simuliidae taxa originating from alpine hydrosystems were tested together with two laboratory nonindigenous culicid taxa. Bioassays indicate that ingestion of 10-month-old decaying leaves from Alnus glutinosa, Populus nigra, and Quercus robur by larvae is more deleterious for Aedes aegypti, A. albopictus, Culex pipiens, Simulium variegatum, and Chironomus annularius than for A. rusticus. Histopathological observations reveal that the midgut epithelium is the main target organ of the toxic effect of dietary leaf litter, which appears to be stronger than that of previously reported tannic acid. There is a general response of the nematocerous larval midgut epithelium to dietary tannins-phenolic compounds: clear cells of the anterior midgut showing symptoms of intoxication before dark cells of the posterior midgut.
研究了落叶对蚊虫发育场所特有的长角亚目双翅目幼虫的相对毒性。对源自高山水系的蚊科、摇蚊科和蚋科类群以及两个实验室非本地蚊科类群进行了测试。生物测定表明，埃及伊蚊、白纹伊蚊、致倦库蚊、杂色斑蚋和环带摇蚊幼虫摄取10个月大的欧洲桤木、黑杨和欧洲栎的腐烂叶片，比对粗腿伊蚊更具有害性。组织病理学观察显示，中肠上皮是膳食落叶毒性作用的主要靶器官，其毒性作用似乎比先前报道的单宁酸更强。长角亚目幼虫中肠上皮对膳食单宁 - 酚类化合物有一般反应：中肠前部的透明细胞比中肠后部的暗细胞更早出现中毒症状。
