Luksiene Z, Kalvelyte A, Supino R
Lithuanian Oncology Centre, Laboratory of Laser and Photodynamic Therapy, Vilnius, Lithuania.
J Photochem Photobiol B. 1999 Sep-Oct;52(1-3):35-42. doi: 10.1016/s1011-1344(99)00098-6.
Ehrlich ascites carcinoma growth and cell damage have been examined after photodynamic therapy (PDT), radiotherapy (RT) and combined treatment. Haematoporphyrin dimethyl ether (HPde) is used as a photosensitizer for PDT and tested as a radiosensitizer for RT. For PDT a non-coherent light source (370 < lambda < 680 nm) equipped with filters is used. gamma-Irradiation consists of 60Co irradiation at a dose of 2 Gy. Both PDT and RT induce a significant delay and inhibition in tumour growth (33 and 38%, respectively). Nevertheless cell damage after these treatments is different: after PDT the cell membrane integrity is damaged and no serious chromosomal aberrations are observed; whereas after gamma-irradiation there is no cell membrane integrity damage, but more significant DNA injuries are observed. It seems evident that HPde is able to act as a photosensitizer as well as a radiosensitizer. Combining PDT and RT produces an additive effect, not dependent on the sequence in which the two treatments are given, when a 1 h time window is used.
在光动力疗法（PDT）、放射疗法（RT）及联合治疗后，对艾氏腹水癌的生长及细胞损伤情况进行了研究。血卟啉二甲醚（HPde）被用作PDT的光敏剂，并作为RT的放射增敏剂进行测试。对于PDT，使用配备滤光片的非相干光源（370＜λ＜680nm）。γ射线照射采用60Co以2Gy的剂量进行照射。PDT和RT均能显著延迟和抑制肿瘤生长（分别为33%和38%）。然而，这些治疗后的细胞损伤情况有所不同：PDT后细胞膜完整性受损，未观察到严重的染色体畸变；而γ射线照射后细胞膜完整性未受损，但观察到更显著的DNA损伤。显然，HPde既能作为光敏剂，也能作为放射增敏剂。当使用1小时的时间窗时，联合PDT和RT会产生相加效应，且该效应不依赖于两种治疗的给予顺序。
