James C D, Davis R, Meyer M, Turner A, Turner S, Withers G, Kam L, Banker G, Craighead H, Isaacson M, Turner J, Shain W
School of Applied and Engineering Physics, Cornell University, Ithaca, NY 14853, USA.
IEEE Trans Biomed Eng. 2000 Jan;47(1):17-21. doi: 10.1109/10.817614.
We describe a method for producing high-resolution chemical patterns on surfaces to control the attachment and growth of cultured neurons. Microcontact printing has been extended to allow the printing of micron-scale protein lines aligned to an underlying pattern of planar microelectrodes. Poly-L-lysine (PL) lines have been printed on the electrode array for electrical studies on cultured neural networks. Rat hippocampal neurons showed a high degree of attachment selectivity to the PL and produced neurites that faithfully grew onto the electrode recording sites.
我们描述了一种在表面生成高分辨率化学图案以控制培养神经元附着和生长的方法。微接触印刷技术已得到扩展，能够印刷与平面微电极底层图案对齐的微米级蛋白质线条。聚-L-赖氨酸（PL）线条已被印刷在电极阵列上，用于对培养神经网络进行电学研究。大鼠海马神经元对PL表现出高度的附着选择性，并产生神经突，这些神经突如实地生长到电极记录位点上。
