Bouwes Bavinck J N, Stark S, Petridis A K, Marugg M E, Ter Schegget J, Westendorp R G, Fuchs P G, Vermeer B J, Pfister H
Departments of Dermatology and Clinical Epidemiology, Leiden University Medical Centre, PO Box 9600, 2300 RC Leiden, The Netherlands.
Br J Dermatol. 2000 Jan;142(1):103-9. doi: 10.1046/j.1365-2133.2000.03248.x.
Epidermodysplasia verruciformis-associated human papillomaviruses (EV-HPVs) are possibly involved in the development of actinic keratoses and may play a part in the pathogenesis of squamous cell carcinoma (SCC) of the skin, as the DNA of these viruses is frequently detected in biopsies of such lesions. Properly designed epidemiological studies, using serological tests to investigate the role of infection with EV-HPVs in cutaneous oncogenesis, are still rare. An IgG-specific enzyme-linked immunosorbent assay using virus-like particles composed of the major capsid protein L1 of the EV-specific HPV 8 (HPV 8 VLPs) was developed and used to test the seroprevalence of HPV 8 in 114 inhabitants of a tropical island, of whom 13 had developed SCC, and 19 had developed basal cell carcinoma. Gender, age, eye and hair colour, sun exposure and number of actinic keratoses were recorded for all individuals. The presence of antibodies against HPV 8 VLPs was associated with the development of large numbers of actinic keratoses. After adjusting for gender, age, eye and hair colour, and sun exposure, the odds ratio to develop 37 (the median in this dataset) or more actinic keratoses in the presence of antibodies against HPV 8 VLPs was 2.3 (95% confidence interval: 1.0; 5.3). Similarly, after adjustment for the same factors, the presence of these antibodies was associated with SCC with an odds ratio of 3.1 (0.74; 13.3), but the small number of individuals with SCC does not permit any definite conclusions. The presence of these antibodies did not appear to be associated with basal cell carcinoma as, after adjustment for the same factors, the odds ratio was 0.73 (0.23; 2.4). This study provides serological evidence that infection with EV-HPVs may play a part in the pathogenesis of actinic keratoses. The role of EV-HPVs in the development of SCC, however, remains to be elucidated.
疣状表皮发育不良相关的人乳头瘤病毒（EV-HPVs）可能与光化性角化病的发生有关，并且可能在皮肤鳞状细胞癌（SCC）的发病机制中起作用，因为在这类病变的活检组织中经常检测到这些病毒的DNA。利用血清学检测来研究EV-HPVs感染在皮肤肿瘤发生中的作用的设计合理的流行病学研究仍然很少。我们开发了一种IgG特异性酶联免疫吸附测定法，该方法使用由EV特异性人乳头瘤病毒8型（HPV 8）的主要衣壳蛋白L1组成的病毒样颗粒，并用于检测一个热带岛屿114名居民中HPV 8的血清阳性率，其中13人患了SCC，19人患了基底细胞癌。记录了所有个体的性别、年龄、眼睛和头发颜色、日晒情况以及光化性角化病的数量。抗HPV 8病毒样颗粒抗体的存在与大量光化性角化病的发生有关。在对性别、年龄、眼睛和头发颜色以及日晒情况进行校正后，存在抗HPV 8病毒样颗粒抗体时发生37例（该数据集中位数）或更多光化性角化病的优势比为2.3（95%置信区间：1.0；5.3）。同样，在对相同因素进行校正后，这些抗体的存在与SCC相关，优势比为3.1（0.74；13.3），但患SCC的个体数量较少，无法得出任何明确结论。这些抗体的存在似乎与基底细胞癌无关，因为在对相同因素进行校正后，优势比为0.73（0.23；2.4）。这项研究提供了血清学证据，表明EV-HPVs感染可能在光化性角化病的发病机制中起作用。然而，EV-HPVs在SCC发生中的作用仍有待阐明。
