Tremousaygue D, Manevski A, Bardet C, Lescure N, Lescure B
Laboratoire de Biologie Moléculaire des Relations Plantes-Microorganismes, CNRS-INRA, BP 27, 31326 Castanet-Tolosan, France.
Plant J. 1999 Dec;20(5):553-61. doi: 10.1046/j.1365-313x.1999.00627.x.
The promoters of Arabidopsis eEF1A genes contain a telomere motif, the telo-box, associated with an activating sequence, the tef-box. Database searches indicated the presence of telo-boxes in the 5' region of numerous genes encoding components of the translational apparatus. By using several promoter constructs we demonstrate that the telo-box is required for the expression of a beta-glucoronidase gene in root primordia of transgenic Arabidopsis. This effect was observed when a telo-box was inserted upstream or downstream from the transcription initiation site, and occurred in synergy with the tef-box. These results clearly indicate that interstitial telomere motifs in plants are involved in control of gene expression. South-western screening of a lambdaZAP library with a double-stranded Arabidopsis telomere motif resulted in characterization of a protein related to the conserved animal protein Puralpha. The possibility of a regulation process similar to that achieved by the Rap1p in Saccharomyces cerevisiae is discussed.
拟南芥eEF1A基因的启动子含有一个端粒基序，即端粒盒，它与一个激活序列，即tef盒相关联。数据库搜索表明，在众多编码翻译装置组件的基因的5'区域存在端粒盒。通过使用几种启动子构建体，我们证明端粒盒是转基因拟南芥根原基中β-葡萄糖醛酸酶基因表达所必需的。当端粒盒插入转录起始位点的上游或下游时观察到这种效应，并且它与tef盒协同作用。这些结果清楚地表明，植物中的间隙端粒基序参与基因表达的控制。用双链拟南芥端粒基序对λZAP文库进行South-western筛选，结果鉴定出一种与保守的动物蛋白Puralpha相关的蛋白质。文中还讨论了存在类似于酿酒酵母中Rap1p所实现的调控过程的可能性。
