Kramer Marianne C, Kim Hee Jong, Palos Kyle R, Garcia Benjamin A, Lyons Eric, Beilstein Mark A, Nelson Andrew D L, Gregory Brian D
Department of Biology, University of Pennsylvania, Philadelphia, PA, United States.
Cell and Molecular Biology Graduate Group, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, United States.
Front Plant Sci. 2022 May 25;13:906603. doi: 10.3389/fpls.2022.906603. eCollection 2022.
Long non-coding RNAs (lncRNAs) are an increasingly studied group of non-protein coding transcripts with a wide variety of molecular functions gaining attention for their roles in numerous biological processes. Nearly 6,000 lncRNAs have been identified in but many have yet to be studied. Here, we examine a class of previously uncharacterized lncRNAs termed BRASSICA RAPA () transcripts that were previously identified for their high level of sequence conservation in the related crop species , their nuclear-localization and protein-bound nature. In particular, we focus on and demonstrate that its abundance is highly tissue and developmental specific, with particularly high levels early in germination. contains two snoRNAs domains within it, making it the first sno-lincRNA example in a non-mammalian system. However, we find that it is processed differently than its mammalian counterparts. We further show that plants lacking display patterns of delayed germination and are overall smaller than wild-type plants. Lastly, we identify the proteins that interact with and propose a novel mechanism of lincRNA action in which it may act as a scaffold with the RACK1A protein to regulate germination and development, possibly through a role in ribosome biogenesis.
长链非编码RNA（lncRNAs）是一类研究日益增多的非蛋白质编码转录本，其具有多种分子功能，因在众多生物学过程中的作用而受到关注。在[具体物种]中已鉴定出近6000种lncRNAs，但许多仍有待研究。在此，我们研究了一类先前未被表征的lncRNAs，称为芸苔属油菜（BRASSICA RAPA）转录本，它们先前因其在相关作物物种中的高度序列保守性、核定位和与蛋白质结合的性质而被鉴定出来。特别是，我们聚焦于[具体转录本名称]，并证明其丰度具有高度的组织和发育特异性，在萌发早期水平尤其高。[具体转录本名称]内部包含两个小分子核仁RNA（snoRNAs）结构域，使其成为非哺乳动物系统中的首个sno-lincRNA实例。然而，我们发现它的加工方式与哺乳动物的对应物不同。我们进一步表明，缺乏[具体转录本名称]的植物表现出萌发延迟的模式，并且总体上比野生型植物小。最后，我们鉴定了与[具体转录本名称]相互作用的蛋白质，并提出了一种lncRNA作用的新机制，即它可能与RACK1A蛋白作为支架来调节萌发和发育，可能是通过在核糖体生物发生中的作用来实现。
