de Jong-Tieben L M, Berkhout R J, ter Schegget J, Vermeer B J, de Fijter J W, Bruijn J A, Westendorp R G, Bouwes Bavinck J N
Department of Dermatology, Leiden University Medical Center, The Netherlands.
Transplantation. 2000 Jan 15;69(1):44-9.
DNA of the epidermodysplasia-verruciformis associated subgroup of HPV (EV-HPV) is frequently detected in biopsies of premalignant lesions and nonmelanoma skin cancers of renal transplant recipients. The prevalence of EV-HPVs, however, has never been systematically studied in benign keratotic skin lesions of patients with or without a history of skin cancer. This study included 42 renal transplant recipients with and 36 without a history of skin cancer. A total of 176 skin biopsies were tested for the presence of EV-HPV DNA, using a nested polymerase chain reaction (PCR).
EV-HPV typing was done by comparison of the sequence of the amplified PCR products with the sequence of all known EV-HPVs. The natural history of the development of keratotic skin lesions was studied. The number of keratotic skin lesions rapidly increased after transplantation. This increase was most pronounced in patients who developed skin cancer. The prevalence of EV-HPV DNA in benign keratotic skin lesions was equally high in patients with and without a history of skin cancer, i.e., 55 and 53% in the two groups, respectively. A large variety of EV-HPV types was found, but of these none were predominantly present in either patient groups. A higher prevalence of EV-HPV DNA was found in benign skin lesions from sun-exposed sites, but only in patients with a history of skin cancer. The association between the number of keratotic skin lesions and the development of skin cancer strongly supports the hypothesis that EV-HPVs play a role in cutaneous oncogenesis. The equally high prevalence of EV-HPV infection in patients with and without a history of skin cancer, however, may indicate that besides EV-HPV infection, other factors, such as sun exposure may also be important.
在肾移植受者的癌前病变和非黑色素瘤皮肤癌活检中，经常检测到与疣状表皮发育不良相关的人乳头瘤病毒亚组（EV-HPV）的DNA。然而，从未对有或没有皮肤癌病史患者的良性角化性皮肤病变中EV-HPV的流行情况进行过系统研究。本研究纳入了42名有皮肤癌病史的肾移植受者和36名无皮肤癌病史的肾移植受者。使用巢式聚合酶链反应（PCR）对总共176份皮肤活检样本进行了EV-HPV DNA检测。
通过将扩增的PCR产物序列与所有已知EV-HPV的序列进行比较来进行EV-HPV分型。研究了角化性皮肤病变发展的自然史。移植后角化性皮肤病变的数量迅速增加。这种增加在发生皮肤癌的患者中最为明显。有和没有皮肤癌病史的患者中，良性角化性皮肤病变中EV-HPV DNA的流行率同样高，两组分别为55%和53%。发现了多种EV-HPV类型，但在任何一组患者中均未发现哪种类型占主导。在暴露于阳光下部位的良性皮肤病变中发现EV-HPV DNA的流行率较高，但仅在有皮肤癌病史的患者中如此。角化性皮肤病变数量与皮肤癌发生之间的关联有力地支持了EV-HPV在皮肤肿瘤发生中起作用的假说。然而，有和没有皮肤癌病史的患者中EV-HPV感染的流行率同样高，这可能表明除了EV-HPV感染外，其他因素，如阳光照射也可能很重要。
