van Der Poel W H, Vinjé J, van Der Heide R, Herrera M I, Vivo A, Koopmans M P
National Institute of Public Health, Bilthoven, The Netherlands.
Emerg Infect Dis. 2000 Jan-Feb;6(1):36-41. doi: 10.3201/eid0601.000106.
Viruses closely related to Norwalk-like viruses (NLVs) were recently found in stored stool samples from two calves (United Kingdom and Germany) and four pigs (Japan), sparking discussions about the potential for zoonotic transmission. To investigate if NLVs are commonly present in farm animals, pooled stool samples from 100 pig farms, 48 chicken farms, 43 dairy cow herds, and 75 veal calf farms from the Netherlands were assayed by reverse transcription- polymerase chain reaction amplification, using primers specific for the detection of NLVs from humans. NLV RNA was detected in 33 (44%) of the specimens from veal calf farms and two (2%) specimens from pig farms. Our data show that NLV infections until recently thought to be restricted to humans occur often in calves and sometimes in pigs. While zoonotic transmission has not been proven, these findings suggest that calves and pigs may be reservoir hosts of NLVs.
最近，在来自两头小牛（英国和德国）和四头猪（日本）的储存粪便样本中发现了与诺如病毒样病毒（NLVs）密切相关的病毒，这引发了关于人畜共患病传播可能性的讨论。为了调查NLVs是否普遍存在于农场动物中，使用针对检测人类NLVs的特异性引物，通过逆转录-聚合酶链反应扩增法对来自荷兰100个养猪场、48个养鸡场、43个奶牛群和75个小牛肉牛场的混合粪便样本进行了检测。在33份（44%）小牛肉牛场样本和2份（2%）养猪场样本中检测到了NLV RNA。我们的数据表明，直到最近还被认为仅限于人类的NLV感染在小牛中经常发生，在猪中有时也会发生。虽然人畜共患病传播尚未得到证实，但这些发现表明小牛和猪可能是NLVs的储存宿主。
