van der Poel W H, Verschoor F, van der Heide R, Herrera M I, Vivo A, Kooreman M, de Roda Husman A M
Microbiological Laboratory for Health Protection, National Institute of Public Health and Environment, Bilthoven, The Netherlands.
Emerg Infect Dis. 2001 Nov-Dec;7(6):970-6. doi: 10.3201/eid0706.010608.
Hepatitis E virus (HEV), a major cause of viral hepatitis in much of the developing world, has recently been detected in swine in North America and Asia, raising concern about potential for zoonotic transmission. To investigate if HEV is commonly present in swine in the Netherlands, pooled stool samples from 115 swine farms and nine individual pigs with diarrhea were assayed by reverse transcription-polymerase chain reaction (RT-PCR) amplification. HEV RNA was detected by RT-PCR and hybridization in 25 (22%) of the pooled specimens, but in none of the individual samples. RT-PCR amplification products of open reading frames 1 and 2 were sequenced, and the results were compared with published sequences of HEV genotypes from humans and swine. HEV strains from swine in the Netherlands were clustered in at least two groups, together with European and American isolates from swine and humans. Our data show that HEV in swine in the Netherlands are genetically closely related to HEV isolates from humans. Although zoonotic transmission has not been proven, these findings suggest that swine may be reservoir hosts of HEV.
戊型肝炎病毒（HEV）是许多发展中国家病毒性肝炎的主要病因，最近在北美和亚洲的猪身上被检测到，这引发了人们对人畜共患病传播可能性的担忧。为了调查荷兰的猪是否普遍感染HEV，我们通过逆转录-聚合酶链反应（RT-PCR）扩增对来自115个猪场的混合粪便样本以及9头腹泻个体猪的样本进行了检测。通过RT-PCR和杂交在25份（22%）混合样本中检测到了HEV RNA，但在任何单个样本中均未检测到。对开放阅读框1和2的RT-PCR扩增产物进行了测序，并将结果与已发表的人类和猪的HEV基因型序列进行了比较。来自荷兰猪的HEV毒株至少聚集在两组中，与来自猪和人类的欧洲及美国分离株在一起。我们的数据表明，荷兰猪体内的HEV在基因上与人类的HEV分离株密切相关。尽管人畜共患病传播尚未得到证实，但这些发现表明猪可能是HEV的储存宿主。
