Chelius M K, Triplett E W
Center for the Study of Nitrogen Fixation, Brock Institute for Environmental Microbiology, and Department of Agronomy, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
Appl Environ Microbiol. 2000 Feb;66(2):783-7. doi: 10.1128/AEM.66.2.783-787.2000.
The endophytic lifestyle of Klebsiella pneumoniae is described, including the production of dinitrogenase reductase by bacteria residing in maize root tissue. The green fluorescent protein (GFP) was used to detect the colonization of maize by K. pneumoniae strains 2028 and 342. These strains were found to reside in intercortical layers of the stem and within the region of maturation in the root. The production of dinitrogenase reductase by GFP-tagged bacteria was visualized using immunolocalization. This activity was only apparent when bacteria were supplied with an exogenous carbon source. The results suggest that maize provides a suitable habitat for K. pneumoniae and that this species is capable of producing nitrogenase under the appropriate plant cultivation conditions.
描述了肺炎克雷伯菌的内生生活方式，包括存在于玉米根组织中的细菌产生二氮还原酶的情况。使用绿色荧光蛋白（GFP）来检测肺炎克雷伯菌菌株2028和342对玉米的定殖。发现这些菌株存在于茎的皮层间层和根的成熟区域内。使用免疫定位观察了绿色荧光蛋白标记细菌产生二氮还原酶的情况。只有在为细菌提供外源碳源时，这种活性才会显现出来。结果表明，玉米为肺炎克雷伯菌提供了适宜的栖息地，并且该物种在适当的植物栽培条件下能够产生固氮酶。
