脂质双层囊泡内外烃链的直接光谱观察。
Direct spectroscopic observation of inner and outer hydrocarbon chains of lipid bilayer vesicles.
作者信息
Longmuir K J, Dahlquist F W
出版信息
Proc Natl Acad Sci U S A. 1976 Aug;73(8):2716-9. doi: 10.1073/pnas.73.8.2716.
Abstract
Distearoylphosphatidylcholines labeled with gem-difluoro groups at selected positions of the fatty acid chains have been prepared. These lipids were incorporated into sonicated vesicles and were examined by fluorine nuclear magnetic resonance spectroscopy. All spectra are characterized by two well-separated fluorine resonances. Paramagnetic ions added to the exterior solution of vesicles of fluorinated lipid broaden the upfield resonances, whereas ions trapped in the vesicles interior broaden the downfield resonances. This demonstrates that the fluorine chemical shift of these compounds is sensitive to differences between the inner and outer monolayers of sonicated vesicles.
摘要
已制备出在脂肪酸链的选定位置标记有偕二氟基团的二硬脂酰磷脂酰胆碱。这些脂质被掺入经超声处理的囊泡中，并通过氟核磁共振光谱进行检测。所有光谱的特征都是两个分离良好的氟共振峰。添加到氟化脂质囊泡外部溶液中的顺磁性离子会使高场共振峰变宽，而捕获在囊泡内部的离子会使低场共振峰变宽。这表明这些化合物的氟化学位移对经超声处理的囊泡内外单层之间的差异敏感。
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