Nosikov V V, Braga E A, Karlishev A V, Zhuze A L, Polyanovsky O L
Nucleic Acids Res. 1976 Sep;3(9):2293-301. doi: 10.1093/nar/3.9.2293.
It is shown here that distamycin A and actinomycin D can protect the recognition sites of endo R.EcoRI, EcoRII, HindII, HindIII, HpaI and HpaII from the attack of these restriction endonucleases. At proper distamycin concentrations only two endo R.EcoRI sites of phage lambda DNA are available for the restriction enzyme--sRI1 and sRI4. This phenomenon results in the appearance of larger DNA fragments comprising several consecutive fragments of endo R.EcoRI complete cleavage. The distamycin fragments isolated from the agarose gels can be subsequently cleaved by endo R.EcoRI with the yield of the fragments of complete digestion. We have compared the effect of distamycin A and actinomycin D on a number of restriction endonucleases having different nucleotide sequences in the recognition sites and established that antibiotic action depends on the nucleotide sequences of the recognition sites and their closest environment
本文表明，偏端霉素A和放线菌素D可以保护内切核酸酶EcoRI、EcoRII、HindII、HindIII、HpaI和HpaII的识别位点免受这些限制性内切酶的攻击。在适当的偏端霉素浓度下，噬菌体λ DNA的仅两个EcoRI内切核酸酶位点可供限制性酶使用——sRI1和sRI4。这种现象导致出现由内切核酸酶EcoRI完全切割的几个连续片段组成的更大DNA片段。从琼脂糖凝胶中分离出的偏端霉素片段随后可以被内切核酸酶EcoRI切割，产生完全消化的片段。我们比较了偏端霉素A和放线菌素D对识别位点具有不同核苷酸序列的多种限制性内切酶的作用，并确定抗生素的作用取决于识别位点及其最接近环境的核苷酸序列。
