Mayer W, Smith A, Fundele R, Haaf T
Max-Planck-Institut für Molekulare Genetik, 14195 Berlin, Germany.
J Cell Biol. 2000 Feb 21;148(4):629-34. doi: 10.1083/jcb.148.4.629.
We have used two different experimental approaches to demonstrate topological separation of parental genomes in preimplantation mouse embryos: mouse eggs fertilized with 5-bromodeoxyuridine (BrdU)-labeled sperm followed by detection of BrdU in early diploid embryos, and differential heterochromatin staining in mouse interspecific hybrid embryos. Separation of chromatin according to parental origin was preserved up to the four-cell embryo stage and then gradually disappeared. In F1 hybrid animals, genome separation was also observed in a proportion of somatic cells. Separate nuclear compartments during preimplantation development, when extreme chromatin remodelling occurs, and possibly in some differentiated cell types, may be associated with epigenetic reprogramming.
用5-溴脱氧尿苷（BrdU）标记的精子使小鼠卵子受精，随后在早期二倍体胚胎中检测BrdU，以及对小鼠种间杂交胚胎进行差异性异染色质染色。根据亲代来源进行的染色质分离在四细胞胚胎阶段之前得以保留，然后逐渐消失。在F1杂种动物中，也在一定比例的体细胞中观察到了基因组分离。在植入前发育过程中，当发生极端的染色质重塑时，以及可能在某些分化细胞类型中，独立的核区室可能与表观遗传重编程有关。
