Asamizu E, Nakamura Y, Sato S, Fukuzawa H, Tabata S
Kazusa DNA Research Institute, Kisarazu, Chiba, Japan.
DNA Res. 1999 Dec 31;6(6):369-73. doi: 10.1093/dnares/6.6.369.
To understand genetic information carried in a unicellular green alga, Chlamydomonas reinhardtii, normalized and size-selected cDNA libraries were constructed from cells at photoautotrophic growth, and a total of 11,571 5'-end sequence tags were established. These sequences were grouped into 3433 independent EST species. Similarity search against the public non-redundant protein database indicated that 817 groups showed significant similarity to registered sequences, of which 140 were of previously identified C. reinhardtii genes, but the remaining 2616 species were novel sequences. The coverage of full-length protein coding regions was estimated to be over 60%. These cDNA clones and EST sequence information will provide a powerful source for the future genome-wide functional analysis of uncharacterized genes.
为了解单细胞绿藻莱茵衣藻中携带的遗传信息，从光合自养生长的细胞构建了标准化且经大小选择的cDNA文库，并建立了总共11571个5'端序列标签。这些序列被分为3433个独立的EST种类。与公共非冗余蛋白质数据库的相似性搜索表明，817个组与已注册序列显示出显著相似性，其中140个是先前鉴定的莱茵衣藻基因，但其余2616个种类是新序列。全长蛋白质编码区的覆盖率估计超过60%。这些cDNA克隆和EST序列信息将为未来对未表征基因进行全基因组功能分析提供强大资源。
