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卵磷脂分散体系中胆固醇分子运动和相分离的核磁共振描述

Nuclear magnetic resonance description of molecular motion and phase separations of cholesterol in lecithin dispersions.

作者信息

Opella S J, Yesinowski J P, Waugh J S

出版信息

Proc Natl Acad Sci U S A. 1976 Nov;73(11):3812-5. doi: 10.1073/pnas.73.11.3812.

DOI:10.1073/pnas.73.11.3812
PMID:1069266
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC431220/
Abstract

Proton-enhanced 13C nuclear magnetic resonance is used to obtain signals from labeled cholesterols in lecithin dispersions. The [26-(13)C]cholesterol resonance indicates that the aliphatic tail of the molecule undergoes reorientation fast enough to average completely the chemical shift anisotropy. In contrast, [4-(13)C]cholesterol signals are characteristic of limited anisotropic reorientation. The resonances from the 4 position are sensitive to the temperature-concentration phase diagram. A phase boundary is observed at about 20 mole percent cholesterol.

摘要

质子增强的13C核磁共振用于从卵磷脂分散体中的标记胆固醇获取信号。[26-(13)C]胆固醇共振表明分子的脂肪族尾部重新取向足够快，足以使化学位移各向异性完全平均化。相比之下，[4-(13)C]胆固醇信号具有有限各向异性重新取向的特征。来自4位的共振对温度-浓度相图敏感。在约20摩尔百分比的胆固醇处观察到一个相界。

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Nuclear magnetic resonance description of molecular motion and phase separations of cholesterol in lecithin dispersions.卵磷脂分散体系中胆固醇分子运动和相分离的核磁共振描述
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本文引用的文献

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Dynamics of lipids in membranes: Heterogeneity and the role of cholesterol.膜中脂质的动力学：异质性与胆固醇的作用
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Molecular motion in spin-labeled phospholipids and membranes.自旋标记磷脂和膜中的分子运动。
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Phase transitions of phospholipid bilayers and membranes of Acholeplasma laidlawii B visualized by freeze fracturing electron microscopy.通过冷冻断裂电子显微镜观察莱氏无胆甾原体B的磷脂双层和细胞膜的相变
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Calorimetric investigation of the influence of cholesterol on the transition properties of bilayers formed from synthetic L- -lecithins in aqueous suspension.量热法研究胆固醇对水悬浮液中合成L-α-卵磷脂形成的双层膜转变特性的影响。
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The stoichiometry and dynamics of lecithin-cholesterol clusters in bilayer membranes.双层膜中卵磷脂 - 胆固醇簇的化学计量学与动力学
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