Möller T E, Gustavsson H F
National Food Administration, Uppsala, Sweden.
J AOAC Int. 2000 Jan-Feb;83(1):99-103.
Fumonisins B1 and B2 were determined in 42 samples of different maize products from the Swedish market by 2 different methods based on cleanup steps using an immunoaffinity column and a combination of SAX + C18 columns, respectively. A simple "precipitation step" was included before the samples were added to the main column(s), giving less column clogging, fewer interfering peaks, and better recoveries for the different sample matrixes. Recovery, repeatability, and results from the survey showed comparable results with the methods. The limit of detection for both methods was 5 micrograms/kg for fumonisin B1 and 10 micrograms/kg for fumonisin B2. All 7 maize chips analyzed and 6 of 8 popcorn samples contained fumonisins (B1 + B2) with averages of 180 and 115 micrograms/kg, respectively. All other samples except a maize flour sample contained little or no fumonisins.
采用两种不同方法，分别基于使用免疫亲和柱和SAX + C18柱组合的净化步骤，对瑞典市场上42种不同玉米产品样本中的伏马菌素B1和B2进行了测定。在将样本添加到主柱之前加入一个简单的“沉淀步骤”，可减少柱堵塞、减少干扰峰，并提高不同样本基质的回收率。回收率、重复性以及调查结果表明这两种方法的结果具有可比性。两种方法对伏马菌素B1的检测限均为5微克/千克，对伏马菌素B2的检测限为10微克/千克。分析的所有7个玉米片样本以及8个爆米花样本中的6个都含有伏马菌素（B1 + B2），平均含量分别为180微克/千克和115微克/千克。除一份玉米粉样本外，所有其他样本含有的伏马菌素很少或不含伏马菌素。
