Li C Q, Pignatelli B, Ohshima H
Unit of Endogenous Cancer Risk Factors, International Agency for Research on Cancer, Lyon, France.
Dig Dis Sci. 2000 Jan;45(1):55-62. doi: 10.1023/a:1005453125433.
The cagA-positive Helicobacter pylori strains are thought to be able to induce interleukin-8 expression and to be associated with gastroduodenal diseases. Inducible nitric oxide synthase (iNOS) may be involved in inflammatory pathogenesis. Our aim was to investigate the interrelationships between cagA and the expression of interleukin-8 and iNOS messenger RNAs, and with the type and degree of inflammation in gastric mucosa. In biopsies from 108 Chinese patients, the cagA, 16S rRNA, interleukin-8, and iNOS mRNAs were analyzed using reverse-transcription polymerase chain reaction. Specimens infected with cagA-positive strains had significantly more severe infiltration by mononuclear and polymorphonuclear leukocytes and more frequently expressed interleukin-8 and iNOS mRNAs than those infected with cagA-negative strains. iNOS and interleukin-8 mRNAs were significantly more frequently expressed together in the specimens with moderate or severe inflammation than in those with normal mucosa or mild inflammation. Our data suggest that interleukin-8 and excess nitric oxide play important roles in the pathogenesis of H. pylori-associated gastroduodenal diseases.
cagA 阳性的幽门螺杆菌菌株被认为能够诱导白细胞介素 -8 的表达，并与胃十二指肠疾病有关。诱导型一氧化氮合酶（iNOS）可能参与炎症发病机制。我们的目的是研究 cagA 与白细胞介素 -8 和 iNOS 信使核糖核酸表达之间的相互关系，以及与胃黏膜炎症的类型和程度的关系。在 108 例中国患者的活检组织中，使用逆转录聚合酶链反应分析了 cagA、16S rRNA、白细胞介素 -8 和 iNOS 信使核糖核酸。与感染 cagA 阴性菌株的标本相比，感染 cagA 阳性菌株的标本中单核细胞和多形核白细胞的浸润明显更严重，白细胞介素 -8 和 iNOS 信使核糖核酸的表达也更频繁。与正常黏膜或轻度炎症的标本相比，iNOS 和白细胞介素 -8 信使核糖核酸在中度或重度炎症标本中共同表达的频率明显更高。我们的数据表明，白细胞介素 -8 和过量的一氧化氮在幽门螺杆菌相关胃十二指肠疾病的发病机制中起重要作用。
