Castro-Rodríguez J A, León-Barúa R, Penny M
Gastrointestinal Physiology Working Group, Cayetano Heredia University, Lima, Peru.
Trans R Soc Trop Med Hyg. 1999 Sep-Oct;93(5):537-9. doi: 10.1016/s0035-9203(99)90372-3.
To investigate the role of Helicobacter pylori in childhood diarrhoea, specific IgG antibodies to H. pylori (determined by an ELISA) were sought in 119 infants aged 3-36 months in Peru. Thirty one of the infants had acute diarrhoea (defined as lasting < 72 h and not present in the previous 3 weeks), 67 had persistent diarrhoea (lasting > or = 14 days with no more than 1 intervening diarrhoea-free day) and the remaining 21 had not had diarrhoea in the previous 3 weeks. The children with diarrhoea had been admitted to hospital in Lima for diarrhoea treatment, and the diarrhoea-free children for investigation of possible tuberculosis. Aspirates of duodenal contents and duplicate stool samples were investigated for the presence of bacterial overgrowth and of pathogenic bacteria, viruses and parasites. Anthropometric measurements were also made. There were no statistically significant differences between the prevalence rates of IgG against H. pylori in the children with acute diarrhoea, persistent diarrhoea and without diarrhoea (32%, 43% and 29%, respectively). In addition, H. pylori infection (as evidenced by specific antibodies) had no apparent influence on the presence of small-bowel overgrowth (in 20% of seropositive children compared with 18% of seronegative children) or of pathogens in the stool (in 53% of seropositive children compared with 49% of seronegative children) or on the occurrence of malnutrition in the groups of children considered as a whole. We conclude that H. pylori infection is not associated with acute or persistent diarrhoeal disease, small-bowel overgrowth, stool pathogens or malnutrition in Peruvian children.
为了研究幽门螺杆菌在儿童腹泻中的作用，我们在秘鲁对119名年龄在3至36个月的婴儿进行了检测，通过酶联免疫吸附测定法（ELISA）来检测他们体内针对幽门螺杆菌的特异性IgG抗体。其中31名婴儿患有急性腹泻（定义为持续时间<72小时且在之前3周内未出现过），67名婴儿患有持续性腹泻（持续时间≥14天且中间无腹泻的间隔天数不超过1天），其余21名婴儿在之前3周内未出现过腹泻。患有腹泻的儿童因腹泻治疗而入住利马的医院，未患腹泻的儿童则是为了排查可能的结核病而接受检查。对十二指肠内容物抽吸物和双份粪便样本进行检测，以确定是否存在细菌过度生长以及病原菌、病毒和寄生虫。同时还进行了人体测量。在患有急性腹泻、持续性腹泻和未患腹泻的儿童中，针对幽门螺杆菌的IgG流行率之间没有统计学上的显著差异（分别为32%、43%和29%）。此外，幽门螺杆菌感染（通过特异性抗体证明）对小肠细菌过度生长（血清阳性儿童中为20%，血清阴性儿童中为18%）、粪便中病原体的存在（血清阳性儿童中为53%，血清阴性儿童中为49%）或整体儿童群体中营养不良的发生情况均无明显影响。我们得出结论，在秘鲁儿童中，幽门螺杆菌感染与急性或持续性腹泻疾病、小肠细菌过度生长、粪便病原体或营养不良无关。
