Haner R L, Llanos W, Mueller L
Varian NMR Systems, 3120 Hansen Way, Palo Alto, California 94304, USA.
J Magn Reson. 2000 Mar;143(1):69-78. doi: 10.1006/jmre.1999.1983.
A detailed characterization of an NMR flow probe for use in direct-injection sample analysis is presented. A 600-MHz, indirect detection NMR flow probe with a 120-microl active volume is evaluated in two configurations: first as a stand-alone small volume probe for the analysis of static, nonflowing solutions, and second as a component in an integrated liquids-handling system used for high-throughput NMR analysis. In the stand-alone mode, (1)H lineshape, sensitivity, radiofrequency (RF) homogeneity, and heat transfer characteristics are measured and compared to conventional-format NMR probes of related design. Commonly used descriptive terminology for the hardware, sample regions, and RF coils are reviewed or defined, and test procedures developed for flow probes are described. The flow probe displayed general performance that is competitive with standard probes. Key advantages of the flow probe include high molar sensitivity, ease of use in an automation setup, and superior reproducibility of magnetic field homogeneity which enables the practical implementation of 1D T2-edited analysis of protein-ligand interactions.
本文介绍了一种用于直接进样样品分析的核磁共振（NMR）流动探头的详细特性。评估了一种具有120微升有效体积的600兆赫兹间接检测NMR流动探头的两种配置：第一种是作为用于分析静态、非流动溶液的独立小体积探头，第二种是作为用于高通量NMR分析的集成液体处理系统的一个组件。在独立模式下，测量了（1）H线形状、灵敏度、射频（RF）均匀性和传热特性，并与相关设计的传统格式NMR探头进行了比较。回顾或定义了硬件、样品区域和RF线圈常用的描述性术语，并描述了为流动探头开发的测试程序。该流动探头显示出与标准探头具有竞争力的一般性能。流动探头的主要优点包括高摩尔灵敏度、易于在自动化设置中使用以及磁场均匀性的卓越再现性，这使得蛋白质-配体相互作用的一维T2编辑分析能够实际应用。
