Department of Chemistry, University of Nebraska-Lincoln, Lincoln, NE 68588, USA.
Anal Biochem. 2011 Sep 15;416(2):234-6. doi: 10.1016/j.ab.2011.05.012. Epub 2011 May 17.
Large-scale nuclear magnetic resonance (NMR) tube cleaning is currently a bottleneck in high-throughput NMR ligand affinity screens. Expensive alternatives include discarding the NMR tubes after a single use (US $2-$8/tube), using commercial NMR tube cleaners ($15,000), and abandoning NMR tubes for flow probe technology ($75,000). Instead, we describe a relatively inexpensive ($400) and easily constructed apparatus that can clean 180 NMR tubes per hour while using a modest amount of solvent. The application of this apparatus significantly shortens the time to recycle NMR tubes while avoiding cross-contamination and tube damage.
大规模核磁共振（NMR）管清洗目前是高通量 NMR 配体亲和力筛选的一个瓶颈。昂贵的替代方案包括单次使用后丢弃 NMR 管（2-8 美元/管）、使用商业 NMR 管清洗器（15000 美元）和改用流探针技术的 NMR 管（75000 美元）。相反，我们描述了一种相对便宜（400 美元）且易于构建的设备，每小时可清洗 180 个 NMR 管，同时使用适量的溶剂。该设备的应用大大缩短了 NMR 管的回收时间，同时避免了交叉污染和管损坏。
