Pradel N, Livrelli V, De Champs C, Palcoux J B, Reynaud A, Scheutz F, Sirot J, Joly B, Forestier C
Groupe de Recherche Pathogénie Bactérienne Intestinale, Faculté de Pharmacie, Université d'Auvergne Clermont-1, Clermont-Ferrand, France.
J Clin Microbiol. 2000 Mar;38(3):1023-31. doi: 10.1128/JCM.38.3.1023-1031.2000.
During a 1-year survey of Shiga toxin-producing Escherichia coli (STEC) prevalence in central France, 2,143 samples were investigated by PCR for Shiga toxin-encoding genes. A total of 330 (70%) of 471 fecal samples collected from healthy cattle at the Clermont-Ferrand slaughterhouse, 47 (11%) of 411 beef samples, 60 (10%) of 603 cheese samples, and 19 (3%) of 658 stool specimens from hospitalized children with and without diarrhea were positive for the stx gene(s). A STEC strain was isolated from 34% (162 of 471) of bovine feces, 4% (16 of 411) of beef samples, 1% (5 of 603) of cheese samples, and 1.5% (10 of 658) of stool specimens. Of the 220 STEC strains isolated, 34 (15%) harbored the stx(1) gene, 116 (53%) harbored the stx(2) gene, and 70 (32%) carried both the stx(1) and stx(2) genes. However, 32 (14.5%) were not cytotoxic for Vero cells. The eae gene, found in 12 (5%) of the 220 strains, was significantly associated with the stx(1) gene and with isolates from children. Sequences homologous to ehxA were found in 102 (46%) of the 220 strains. Thirteen serotypes, OX3:H2, O113:H21, O113:H4, OX3:H21, O6:H10, OX178:H19, O171:H2, O46:H38, O172:H21, O22:H16, O91:H10, O91:H21, and O22:H8, accounted for 102 (55%) of 186 typeable isolates, and only one strain (0.5% of the 186 STEC isolates from cattle), belonged to the O157:H7 serotype. We showed that the majority of the STEC isolates from cattle, beef, and cheese were not likely to be pathogenic for humans and that the STEC strains isolated from children in this study were probably not responsible for diarrheal disease. Finally, the strains associated with hemolytic-uremic syndrome in the same geographical area were shown to belong to particular subsets of the STEC population found in the bovine reservoir.
在对法国中部产志贺毒素大肠杆菌（STEC）流行情况进行的为期1年的调查中，通过聚合酶链反应（PCR）检测了2143份样本中的志贺毒素编码基因。从克莱蒙费朗屠宰场的健康牛采集的471份粪便样本中，共有330份（70%）呈stx基因阳性；411份牛肉样本中有47份（11%）呈阳性；603份奶酪样本中有60份（10%）呈阳性；658份住院腹泻和非腹泻儿童的粪便标本中有19份（3%）呈阳性。从471份牛粪便样本中的34%（162份）、411份牛肉样本中的4%（16份）、603份奶酪样本中的1%（5份）以及658份粪便标本中的1.5%（10份）中分离出了STEC菌株。在分离出的220株STEC菌株中，34株（15%）携带stx(1)基因，116株（53%）携带stx(2)基因，70株（32%）同时携带stx(1)和stx(2)基因。然而，32株（14.5%）对Vero细胞无细胞毒性。在220株菌株中的12株（5%）中发现了eae基因，该基因与stx(1)基因以及来自儿童的分离株显著相关。在220株菌株中的102株（46%）中发现了与ehxA同源的序列。13种血清型，即OX3:H2、O113:H21、O113:H4、OX3:H21、O6:H10、OX178:H19、O171:H2、O46:H38、O172:H21、O22:H16、O91:H10、O91:H21和O22:H8，占186株可分型分离株中的102株（55%），只有1株（来自牛的186株STEC分离株中的0.5%）属于O157:H7血清型。我们发现，从牛、牛肉和奶酪中分离出的大多数STEC分离株对人类致病的可能性不大，并且本研究中从儿童分离出的STEC菌株可能与腹泻疾病无关。最后，在同一地理区域与溶血尿毒综合征相关的菌株被证明属于在牛源中发现的STEC群体的特定亚群。
