Redlich A, Müller W A
O.-V.-Guericke Universität Magdeburg, Medizinische Fakultät, Institut für Medizinische Mikrobiologie, Bereich Medizinische Parasitologie, Germany.
Parasitol Res. 1998 Sep;84(9):700-6. doi: 10.1007/s004360050473.
We developed an indirect enzyme-linked immunosorbent assay (ELISA) for the serological diagnosis of acute toxoplasmosis that used the recombinant granule antigen GRA6-GST as diagnostic antigen for the detection of IgG antibodies to Toxoplasma gondii in human sera. A total of 431 sera obtained from 336 patients with acute and chronic toxoplasmosis and from patients who were not infected with T. gondii were tested. Sera from patients with acute T. gondii infection, chronic infection, and no infection showed different absorbance values. For discrimination between the presence and the absence of acute toxoplasmosis the assay reached a specificity of 99.6%. Only one of the sera without significant anti-T. gondii. IgM antibodies showed a positive reaction to rGRA6-GST. The assay showed good intra- and interassay reproducibility (CV 6%/14%). We included a glutathione S-transferase (GST)-IgG enzyme immunoassay as a control assay in this study. Only 7 (4%) of 159 random sample sera reacted positively with GST.
我们开发了一种用于急性弓形虫病血清学诊断的间接酶联免疫吸附测定（ELISA），该方法使用重组颗粒抗原GRA6-GST作为诊断抗原，用于检测人血清中针对刚地弓形虫的IgG抗体。共检测了从336例急性和慢性弓形虫病患者以及未感染刚地弓形虫的患者中获得的431份血清。急性刚地弓形虫感染患者、慢性感染患者和未感染患者的血清显示出不同的吸光度值。对于区分急性弓形虫病的有无，该测定的特异性达到99.6%。在无显著抗刚地弓形虫IgM抗体的血清中，只有一份对rGRA6-GST呈阳性反应。该测定显示出良好的批内和批间重复性（变异系数分别为6%/14%）。在本研究中，我们纳入了谷胱甘肽S-转移酶（GST）-IgG酶免疫测定作为对照测定。159份随机抽样血清中只有7份（4%）与GST呈阳性反应。
