Eggert F M, Germain J P
Histochemistry. 1979 Jan 22;59(3):215-24. doi: 10.1007/BF00495669.
The demineralization of routine histological specimens in buffers of weakly ionized organic acids, unbuffered formic acid, and EDTA was investigated. The rate of demineralization was measured by a chemical method and from radiographs. Lactate-containing buffers and buffers of formic acid with its potassium salt were more rapid in effect than any other agent. Acidic buffers and unbuffered formic acid produced rapid diffuse demineralization with secondary precipitation of calcium salts. Preservation of dental enamel in such buffers resulted from the significantly slower rate of enamel demineralization than that for bone and dentine. In rapid demineralizing agents the secondary salts were quickly redissolved while in slow buffers these salts persisted. Multivalent ions such as citrate and maleate slowed the rate of demineralization, and a citrate-containing buffer was the slowest of all the agents tested. Demineralization in EDTA exhibited a different pattern with the establishment of a well-defined front of demineralization without apparent reprecipitation. EDTA attacked enamel, bone and dentine at the same rate. An attempt was made to relate the observed rates of demineralization to current theories of the demineralization process.
研究了常规组织学标本在弱电离有机酸缓冲液、未缓冲的甲酸和乙二胺四乙酸（EDTA）中的脱矿情况。通过化学方法和X光片测量脱矿速率。含乳酸的缓冲液和甲酸及其钾盐的缓冲液比其他任何试剂的脱矿效果都更快。酸性缓冲液和未缓冲的甲酸会产生快速的弥漫性脱矿，并伴有钙盐的二次沉淀。在这类缓冲液中牙釉质得以保存，是因为牙釉质的脱矿速率明显慢于骨和牙本质。在快速脱矿剂中，二次盐会迅速重新溶解，而在慢速缓冲液中这些盐会持续存在。柠檬酸和马来酸等多价离子会减缓脱矿速率，含柠檬酸盐的缓冲液是所有测试试剂中脱矿最慢的。EDTA脱矿呈现出不同的模式，形成了明确的脱矿前沿，且没有明显的再沉淀现象。EDTA对牙釉质、骨和牙本质的侵蚀速率相同。人们试图将观察到的脱矿速率与当前的脱矿过程理论联系起来。
