Knutson M D, Handelman G J, Viteri F E
Department of Nutrition, Harvard School of Public Health, Boston, MA 02115, USA.
Free Radic Biol Med. 2000 Feb 15;28(4):514-9. doi: 10.1016/s0891-5849(99)00230-0.
Numerous studies in animals and humans provide evidence that ethane and pentane in expired air are useful markers of in vivo lipid peroxidation. The measurement of breath hydrocarbons, being noninvasive, is well suited for routine use in research and clinical settings. However, the lack of standardized methods for collecting, processing, and analyzing expired air has resulted in the use of a wide variety of different methods that have yielded highly disparate results among investigators. This review outlines the methods that we have developed and validated for measuring ethane and pentane in expired air from rats and humans. We describe the advantages of these methods, their performance, as well as potential errors that can be introduced during sample collection, concentration, and analysis. A main source of error involves contamination with ambient-air ethane and pentane, the concentrations of which are usually much greater and more variable than those in expired air. Thus, it appears that the effective removal of ambient-air hydrocarbons from the subject's lungs before collection is an important step in standardizing the collection procedure. Also discussed is whether ethane or pentane is a better marker of in vivo lipid peroxidation.
众多针对动物和人类的研究表明，呼出气体中的乙烷和戊烷是体内脂质过氧化的有用标志物。呼出碳氢化合物的测量具有非侵入性，非常适合在研究和临床环境中常规使用。然而，由于缺乏收集、处理和分析呼出气体的标准化方法，导致使用了各种各样不同的方法，研究人员得到的结果差异很大。这篇综述概述了我们已开发并验证的用于测量大鼠和人类呼出气体中乙烷和戊烷的方法。我们描述了这些方法的优点、性能，以及在样品收集、浓缩和分析过程中可能引入的潜在误差。一个主要误差来源涉及环境空气中乙烷和戊烷的污染，其浓度通常比呼出气体中的浓度高得多且变化更大。因此，在收集前有效去除受试者肺部的环境空气碳氢化合物似乎是标准化收集程序的重要一步。还讨论了乙烷或戊烷是否是体内脂质过氧化的更好标志物。
