Wong H C, Chen M C, Liu S H, Liu D P
Department of Microbiology, Soochow University, Taipei, Taiwan, ROC.
Int J Food Microbiol. 1999 Nov 15;52(3):181-8. doi: 10.1016/s0168-1605(99)00143-9.
Vibrio parahaemolyticus is an important pathogen of humans and aquacultured animals, especially in Asian countries. In this study, we examined 686 samples of seafood imported from Hong Kong, Indonesia, Thailand and Vietnam for V. parahaemolyticus. V. parahaemolyticus was recovered from 315 (45.9%) samples. The incidence of V. parahaemolyticus in products from Hong Kong and Thailand was markedly higher than the incidence in products from Indonesia and Vietnam. The incidence rates in shrimp, crab, snail, lobster, sand crab, fish and crawfish were 75.8, 73.3, 44.3, 44.1, 32.5, 29.3 and 21.1%, respectively. None of the isolates possessed the hemolysin genes (tdh, trh). The chromosomal DNA of 121 randomly selected imported isolates and three local environmental strains was digested with SfiI and analyzed by pulsed-field gel electrophoresis (PFGE). These isolates were then grouped into 96 different but mostly unique PFGE patterns. After hierarchical cluster analysis, these patterns could be arbitrarily grouped into twenty-two PFGE types (type A to V). A wide range of PFGE types were identified in isolates from different origin. Moreover, the PFGE types were not specifically associated with the origin or kind of seafood. These results reveal the high genetic diversity in V. parahaemolyticus isolated from seafood.
副溶血性弧菌是人和水产养殖动物的重要病原菌，在亚洲国家尤为如此。在本研究中，我们检测了从香港、印度尼西亚、泰国和越南进口的686份海鲜样本中的副溶血性弧菌。从315份（45.9%）样本中分离出了副溶血性弧菌。香港和泰国产品中副溶血性弧菌的发生率明显高于印度尼西亚和越南产品中的发生率。虾、蟹、蜗牛、龙虾、招潮蟹、鱼和小龙虾中的发生率分别为75.8%、73.3%、44.3%、44.1%、32.5%、29.3%和21.1%。所有分离株均不具有溶血素基因（tdh、trh）。用SfiI消化121株随机选择的进口分离株和3株本地环境菌株的染色体DNA，并通过脉冲场凝胶电泳（PFGE）进行分析。这些分离株随后被分为96种不同但大多独特的PFGE模式。经过层次聚类分析，这些模式可被任意分为22种PFGE类型（A至V型）。在来自不同产地的分离株中鉴定出了广泛的PFGE类型。此外，PFGE类型与海鲜的产地或种类没有特定关联。这些结果揭示了从海鲜中分离出的副溶血性弧菌具有高度的遗传多样性。
