Tan Chia W, Malcolm Tan T H, Kuan Chee H, Thung Tze Y, Chang Wei S, Loo Yuet Y, Premarathne Jayasekara M K J K, Ramzi Othman B, Norshafawatie Mohd F S, Yusralimuna Nordin, Rukayadi Yaya, Nakaguchi Yoshitsugu, Nishibuchi Mitsuaki, Radu Son
Department of Food Science, Faculty of Food Science and Technology, Universiti Putra MalaysiaSelangor, Malaysia.
Department of Livestock and Avian Science, Faculty of Livestock, Fisheries and Nutrition, Wayamba University of Sri LankaMakandura, Sri Lanka.
Front Microbiol. 2017 Jun 13;8:1087. doi: 10.3389/fmicb.2017.01087. eCollection 2017.
Numerous prevalence studies and outbreaks of infection have been extensively reported in shellfish and crustaceans. Information on the quantitative detection of in finfish species is limited. In this study, short mackerels () obtained from different retail marketplaces were monitored with the presence of total and pathogenic strains of . Out of 130 short mackerel samples, 116 (89.2%) were detected with the presence of total and microbial loads of total ranging from <3 to >10 MPN/g. Prevalence of total was found highest in wet markets (95.2%) followed by minimarkets (89.1%) and hypermarkets (83.3%). Pathogenic strains (+ and/or +) were detected in 16.2% (21 of 130) of short mackerel samples. The density of + strains were examined ranging from 3.6 to >10 MPN/g and microbial loads of strains positive for both and were found ranging from 300 to 740 MPN/g. On the other hand, antibiotic susceptibility profiles of strains isolated from short mackerels were determined through disc diffusion method in this study. Assessment of antimicrobial susceptibility profile of revealed majority of the isolates were highly susceptible to ampicillin sulbactam, meropenem, ceftazidime, and imipenem, but resistant to penicillin G and ampicillin. Two isolates (2.99%) exhibited the highest multiple antibiotic resistance (MAR) index value of 0.41 which shown resistance to 7 antibiotics. Results of the present study demonstrated that the occurrence of pathogenic strains in short mackerels and multidrug resistance of isolates could be a potential public health concerns to the consumer. Furthermore, prevalence data attained from the current study can be further used to develop a microbial risk assessment model to estimate health risks associated with the consumption of short mackerels contaminated with pathogenic .
在贝类和甲壳类动物中，大量的患病率研究和感染爆发情况已有广泛报道。关于在鱼类中进行定量检测的信息有限。在本研究中，对从不同零售市场获取的竹荚鱼进行了副溶血性弧菌总菌株和致病菌株的监测。在130份竹荚鱼样本中，116份（89.2%）检测到副溶血性弧菌总菌株，其微生物负荷总量范围为<3至>10 MPN/g。副溶血性弧菌总患病率在湿货市场最高（95.2%），其次是小型市场（89.1%）和大型超市（83.3%）。在16.2%（130份中的21份）的竹荚鱼样本中检测到致病副溶血性弧菌菌株（tdh+和/或trh+）。tdh+菌株的密度检测范围为3.6至>10 MPN/g，同时检测到tdh和trh均呈阳性的菌株的微生物负荷范围为300至740 MPN/g。另一方面，本研究通过纸片扩散法测定了从竹荚鱼中分离出的副溶血性弧菌菌株的抗生素敏感性谱。对副溶血性弧菌抗菌敏感性谱的评估表明，大多数分离株对氨苄西林舒巴坦、美罗培南、头孢他啶和亚胺培南高度敏感，但对青霉素G和氨苄西林耐药。有两个分离株（2.99%）表现出最高的多重耐药（MAR）指数值0.41，对7种抗生素耐药。本研究结果表明，竹荚鱼中致病副溶血性弧菌菌株的出现以及分离株的多重耐药性可能对消费者构成潜在的公共卫生问题。此外，从本研究获得的患病率数据可进一步用于建立微生物风险评估模型，以估计食用受致病副溶血性弧菌污染的竹荚鱼相关的健康风险。
