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NPIRL区域中异亮氨酸和亮氨酸的大烷基侧链构成了孢粉素前体液泡分选决定簇的核心。

Large alkyl side-chains of isoleucine and leucine in the NPIRL region constitute the core of the vacuolar sorting determinant of sporamin precursor.

作者信息

Matsuoka K, Nakamura K

机构信息

Laboratory of Biochemistry, Graduate School of Bioagricultural Sciences, Nagoya University, Japan.

出版信息

Plant Mol Biol. 1999 Dec;41(6):825-35. doi: 10.1023/a:1006357413084.

DOI:10.1023/a:1006357413084
PMID:10737147
Abstract

The N-terminal propeptide of the sporamin precursor contains vacuolar targeting information within the Asn-26/Pro-27/Ile-28/Arg-29/Leu-30 (NPIRL) sequence. An Agrobacterium-mediated transient expression assay with tobacco BY-2 cells was employed to investigate the role of each amino acid of the NPIRL region in vacuolar targeting. Replacement of Asn-26, Pro-27, Ile-28 and Leu-30 with several amino acids caused secretion of the mutant prosporamin. Leu was the only amino acid that could be substituted for Ile-28 without affecting transport. Exchange of Leu-30 for amino acids with small side-chains abolished vacuolar delivery. These results indicate that the consensus composition of the NPIRL sequence is [preferably Asn]-[not acidic]-[Ile or Leu]-[any amino acid]-[large and hydrophobic] and suggest that the large alkyl side-chains of Ile-28 and Leu-30 constitute the core of the vacuolar sorting determinant.

摘要

孢粉素前体的N端前肽在天冬酰胺-26/脯氨酸-27/异亮氨酸-28/精氨酸-29/亮氨酸-30（NPIRL）序列中包含液泡靶向信息。利用农杆菌介导的烟草BY-2细胞瞬时表达试验，研究NPIRL区域每个氨基酸在液泡靶向中的作用。用几种氨基酸替换天冬酰胺-26、脯氨酸-27、异亮氨酸-28和亮氨酸-30会导致突变型前孢粉素的分泌。亮氨酸是唯一可以替代异亮氨酸-28而不影响转运的氨基酸。将亮氨酸-30替换为侧链小的氨基酸会消除液泡运输。这些结果表明，NPIRL序列的共有组成是[最好是天冬酰胺]-[非酸性]-[异亮氨酸或亮氨酸]-[任何氨基酸]-[大的疏水性氨基酸]，并表明异亮氨酸-28和亮氨酸-30的大烷基侧链构成了液泡分选决定簇的核心。

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