Kiparisov Sergey, Petrov Alexey, Meskauskas Arturas, Sergiev Petr V, Dontsova Olga A, Dinman Jonathan D
Department of Chemistry, Moscow State University, Russia.
Mol Genet Genomics. 2005 Oct;274(3):235-47. doi: 10.1007/s00438-005-0020-9. Epub 2005 Oct 20.
5S rRNA extends from the central protuberance of the large ribosomal subunit, through the A-site finger, and down to the GTPase-associated center. Here, we present a structure-function analysis of seven 5S rRNA alleles which are sufficient for viability in the yeast Saccharomyces cerevisiae when expressed in the absence of wild-type 5S rRNAs, and extend this analysis using a large bank of mutant alleles that show semi-dominant phenotypes in the presence of wild-type 5S rRNA. This analysis supports the hypothesis that 5S rRNA serves to link together several different functional centers of the ribosome. Data are also presented which suggest that in eukaryotic genomes selection has favored the maintenance of multiple alleles of 5S rRNA, and that these may provide cells with a mechanism to post-transcriptionally regulate gene expression.
5S核糖体RNA从大核糖体亚基的中央突起延伸，穿过A位点指状结构，一直延伸到GTP酶相关中心。在此，我们对七个5S核糖体RNA等位基因进行了结构-功能分析，当在没有野生型5S核糖体RNA的情况下表达时，这些等位基因足以维持酿酒酵母的生存能力，并使用一大组在野生型5S核糖体RNA存在时表现出半显性表型的突变等位基因扩展了这一分析。该分析支持了5S核糖体RNA用于将核糖体的几个不同功能中心连接在一起的假说。还提供了数据表明，在真核生物基因组中，选择有利于维持5S核糖体RNA的多个等位基因，并且这些等位基因可能为细胞提供一种转录后调节基因表达的机制。
