Dojka M A, Harris J K, Pace N R
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado 80309-0347, USA.
Appl Environ Microbiol. 2000 Apr;66(4):1617-21. doi: 10.1128/AEM.66.4.1617-1621.2000.
Culture-independent molecular phylogenetic methods were used to explore the breadth of diversity and environmental distribution of members of the division-level "candidate" phylogenetic group WS6, recently discovered in a contaminated aquifer and with no cultivated representatives. A broad diversity of WS6-affiliated sequences were cloned from 7 of 12 environments investigated: mainly from anaerobic sediment environments. The number of sequences representing the WS6 candidate division was increased from 3 to 60 in this study. The extent of phylogenetic divergence (sequence difference) in this candidate division was found to be among the largest of any known bacterial division. This indicates that organisms representing the WS6 phylogenetic division offer a broad diversity of undiscovered biochemical and metabolic novelty. These results provide a framework for the further study of these evidently important kinds of organisms and tools, the sequences, with which to do so.
不依赖培养的分子系统发育方法被用于探索分类水平的“候选”系统发育组WS6成员的多样性广度和环境分布，该组最近在一个受污染的含水层中被发现，且尚无培养的代表菌株。从所调查的12个环境中的7个环境中克隆出了与WS6相关的多种序列：主要来自厌氧沉积物环境。在本研究中，代表WS6候选分类的序列数量从3个增加到了60个。发现该候选分类中的系统发育分歧程度（序列差异）在所有已知细菌分类中是最大的之一。这表明代表WS6系统发育分类的生物体具有广泛多样的未被发现的生化和代谢新奇性。这些结果为进一步研究这些显然重要的生物体类型以及用于研究的工具——序列，提供了一个框架。
