Cifuentes A, Antón J, Benlloch S, Donnelly A, Herbert R A, Rodríguez-Valera F
División de Microbiología, Universidad Miguel Hernández, Campus de San Juan, 03550 San Juan, Alicante, Spain.
Appl Environ Microbiol. 2000 Apr;66(4):1715-9. doi: 10.1128/AEM.66.4.1715-1719.2000.
The diversity of microorganisms present in a sediment colonized by the phanerogam Zostera noltii has been analyzed. Microbial DNA was extracted and used for constructing two 16S rDNA clone libraries for Bacteria and Archaea. Bacterial diversity was very high in these samples, since 57 different sequences were found among the 60 clones analyzed. Eight major lineages of the Domain Bacteria were represented in the library. The most frequently retrieved bacterial group (36% of the clones) was delta-Proteobacteria related to sulfate-reducing bacteria. The second most abundant group (27%) was gamma-Proteobacteria, including five clones closely related to S-oxidizing endosymbionts. The archaeal clone library included members of Crenarchaeota and Euryarchaeota, with nine different sequences among the 15 analyzed clones, indicating less diversity when compared to the Bacteria organisms. None of these sequences was closely related to cultured Archaea organisms.
对由显花植物波喜荡草定殖的沉积物中存在的微生物多样性进行了分析。提取了微生物DNA，并用于构建细菌和古菌的两个16S rDNA克隆文库。这些样本中的细菌多样性非常高，因为在分析的60个克隆中发现了57个不同的序列。文库中代表了细菌域的八个主要谱系。检索到的最常见细菌类群（占克隆的36%）是与硫酸盐还原菌相关的δ-变形菌纲。第二丰富的类群（占27%）是γ-变形菌纲，包括五个与硫氧化内共生体密切相关的克隆。古菌克隆文库包括泉古菌门和广古菌门的成员，在分析的15个克隆中有九个不同的序列，表明与细菌生物相比多样性较低。这些序列中没有一个与培养的古菌生物密切相关。
