Limia A, Alarcón T, Jiménez M L, López-Brea M
Department of Clinical Microbiology, Hospital Universitario de la Princesa, Madrid, Spain.
Eur J Clin Microbiol Infect Dis. 2000 Feb;19(2):128-31. doi: 10.1007/s100960050444.
A collection of 180 clinical isolates of Streptococcus milleri group were identified to species level using two phenotypic methods (a commercial system and the reference method based on differential phenotypic reactions) and a genotypic method (hybridisation of the 16S rRNA gene with species-specific probes) in order to evaluate the performance of the respective methods. A high level of agreement (80%) was observed between the results of the reference method and the genotypic method. The highest level of agreement was found for the species Streptococcus anginosus (83%), a high level of agreement (76%) also being achieved for Streptococcus constellatus and Streptococcus intermedius. The sensitivity of the commercial system compared to the genotypic method was 76% overall, but it was low (57.5%) for Streptococcus intermedius. Twenty-five strains belonged to the recently described CI strains.
使用两种表型方法（一种商业系统和基于差异表型反应的参考方法）以及一种基因型方法（16S rRNA基因与种特异性探针杂交），对180株米勒链球菌群临床分离株进行种水平鉴定，以评估各方法的性能。参考方法和基因型方法的结果之间观察到高度一致性（80%）。对于咽峡炎链球菌，一致性水平最高（83%），星座链球菌和中间链球菌也达到了较高的一致性水平（76%）。与基因型方法相比，商业系统的总体敏感性为76%，但对于中间链球菌则较低（57.5%）。25株菌株属于最近描述的CI菌株。
