Ho J W, Liang R H, Srivastava G
Department of Pathology, University of Hong Kong, People's Republic of China.
Mol Pathol. 1999 Oct;52(5):269-74. doi: 10.1136/mp.52.5.269.
To investigate whether specific cytokines are secreted locally at the tumour site in Epstein-Barr virus (EBV) positive peripheral T cell lymphoma (PTCL).
An RNase protection assay system was used to study the differential expression of 21 cytokines in parallel in eight cases of EBV positive non-nasal PTCL, and compared with 11 EBV negative non-nasal PTCLs and three EBV positive nasal natural killer (NK) cell lymphomas.
Among the eight EBV positive cases, interferon gamma (IFN-gamma), lymphotoxin beta (LT beta), interleukin 10 (IL-10), tumour necrosis factor alpha (TNF-alpha), transforming growth factor beta 1 (TGF-beta 1), and IL-1 receptor a (IL-Ra) were frequently detectable. IL-15, IL-6, IL-4, IL-1 beta, TNF-beta, and IL-9 were sporadically detectable. Of the frequently detectable cytokines, IFN-gamma and LT beta were commonly detected in the EBV negative cases. For cases with > 50% EBV encoded small non-polyadenylated RNA (EBER) positive cells, IL-10, TNF-alpha, and TGF-beta 1 were detected in three of three cases, and IL-1Ra in two of three cases. For cases with < 20% EBER positive cells, IL-10 was detected in three of five cases, TNF-alpha in two of four cases, but TGF-beta 1 and IL-1Ra were not detected. Interestingly, IL-6 was detected in two of three cases with > 50% EBER positive cells, but only in one of five cases with < 20% EBER positive cells. For comparison, in NK cell lymphomas, IL-10, TNF-alpha, IL-1Ra, and IL-6 were all detectable, but TGF-beta 1 was not detected at all. Immunohistochemical staining revealed IL-10 in many cells; in contrast, EBV latent membrane protein 1 (LMP1) was only found to be positive in isolated cells.
Certain cytokines, such as IL-10 and TNF-alpha, might be expressed preferentially in EBV positive peripheral T cell lymphomas. It is likely that such a cytokine environment enhances EBV infection and contributes towards tumorigenesis.
研究在爱泼斯坦-巴尔病毒（EBV）阳性外周T细胞淋巴瘤（PTCL）中特定细胞因子是否在肿瘤部位局部分泌。
采用核糖核酸酶保护分析系统，对8例EBV阳性非鼻型PTCL中21种细胞因子的差异表达进行平行研究，并与11例EBV阴性非鼻型PTCL和3例EBV阳性鼻型自然杀伤（NK）细胞淋巴瘤进行比较。
在8例EBV阳性病例中，经常可检测到干扰素γ（IFN-γ）、淋巴毒素β（LTβ）、白细胞介素10（IL-10）、肿瘤坏死因子α（TNF-α）、转化生长因子β1（TGF-β1）和IL-1受体α（IL-Rα）。IL-15、IL-6、IL-4、IL-1β、TNF-β和IL-9偶尔可检测到。在经常可检测到的细胞因子中，IFN-γ和LTβ在EBV阴性病例中也普遍检测到。对于EBV编码的小非聚腺苷酸化RNA（EBER）阳性细胞>50%的病例，3例中有3例检测到IL-10、TNF-α和TGF-β1，3例中有2例检测到IL-Rα。对于EBER阳性细胞<20%的病例，5例中有3例检测到IL-10，4例中有2例检测到TNF-α，但未检测到TGF-β1和IL-Rα。有趣的是，在EBER阳性细胞>50%的3例病例中有2例检测到IL-6，但在EBER阳性细胞<20%的5例病例中只有1例检测到。作为对照，在NK细胞淋巴瘤中，IL-10、TNF-α、IL-Rα和IL-6均能检测到，但完全未检测到TGF-β1。免疫组织化学染色显示许多细胞中有IL-10；相反，EBV潜伏膜蛋白1（LMP1）仅在单个细胞中呈阳性。
某些细胞因子，如IL-10和TNF-α，可能在EBV阳性外周T细胞淋巴瘤中优先表达。这样的细胞因子环境可能会增强EBV感染并促进肿瘤发生。
