Taus C, Pucci E, Cartechini E, Fié A, Giuliani G, Clementi M, Menzo S
Istituto Malattie del Sistema Nervoso, Università di Ancona, Italy.
Acta Neurol Scand. 2000 Apr;101(4):224-8. doi: 10.1034/j.1600-0404.2000.101004224.x.
To contribute to clarifying the controversy on the association between Human Herpesviruses 6 and 7 (HHV-6, HHV-7) and multiple sclerosis (MS) studying patients with relapsing-remitting MS (RRMS) with or without evidence of disease activity (clinically or radiologically evaluated).
In 25 RRMS patients, 7 suspected MS patients and 9 patients with other neurological diseases, the following parameters were analysed: i) antibody titers (IgM and IgG) against HHV-6 by indirect immunofluorescence both in serum and cerebrospinal fluid (CSF) samples; ii) PCR-detection of HHV-6 DNA and HHV-7 DNA in CSF and HHV-6 DNA in peripheral blood mononuclear cells (PBMCs). MS patients in remission underwent a gadolinium-enhanced magnetic resonance imaging in proximity of sample collections.
No viral DNA was found in any CSF sample, HHV-6 DNA frequency in PBMCs of MS patients and controls was not statistically different. Antibody titers against HHV-6 were comparable to those of the general population. Some 30.4% of MS patients were seronegative to HHV6.
Our data suggest that there is no relationship between HHV-6 or HHV-7 and MS.
通过研究复发缓解型多发性硬化症（RRMS）患者有无疾病活动证据（临床或影像学评估），以助于澄清人类疱疹病毒6型和7型（HHV-6、HHV-7）与多发性硬化症（MS）之间关联的争议。
对25例RRMS患者、7例疑似MS患者和9例患有其他神经系统疾病的患者，分析以下参数：i）血清和脑脊液（CSF）样本中通过间接免疫荧光法检测抗HHV-6的抗体滴度（IgM和IgG）；ii）CSF中HHV-6 DNA和HHV-7 DNA的PCR检测以及外周血单个核细胞（PBMCs）中HHV-6 DNA的检测。缓解期的MS患者在样本采集前后进行了钆增强磁共振成像检查。
在任何CSF样本中均未发现病毒DNA，MS患者和对照组PBMCs中HHV-6 DNA的频率无统计学差异。抗HHV-6的抗体滴度与普通人群相当。约30.4%的MS患者对HHV6血清学阴性。
我们的数据表明HHV-6或HHV-7与MS之间无关联。
