Wang Y, Go V, Holland J F, Melana S M, Pogo B G
Department of Pathology, Mount Sinai School of Medicine, New York, New York 10029, USA.
Clin Cancer Res. 1998 Oct;4(10):2565-8.
We have reported previously (Wang et al, Cancer Res., 55: 5173-5179, 1995) the presence of a 660-bp sequence that is 90-98% homologous to the env gene of mouse mammary tumor virus in 38% of 314 unselected breast cancer samples, in some breast cancer cell lines, and in 1.8% of the 107 normal breast specimens from reduction mammoplasties. In this communication, we have investigated whether the 660-bp sequence or a smaller 256-bp sequence within the 660 bp were expressed in breast tumors, normal breasts, and breast cancer cell lines by means of reverse transcription-PCR. The results indicated that expression of the 660-bp sequence was detected in 66% of the sequence-positive breast tumors studied and in none of the negative tumors or normal breasts and that the 256-bp sequence was expressed in all of the env-positive breast cancer cell lines tested. Sequence analysis revealed that the expressed 660-bp sequence is 98% homologous to the env gene of mouse mammary tumor virus with only one small stretch of 46 bp homologous to endogenous viral sequences. The presence and expression of this sequence is related to a group of human breast cancers in which the concept of a retroviral etiology can be entertained.
我们之前已经报道过（Wang等人，《癌症研究》，55: 5173 - 5179, 1995），在314份未经筛选的乳腺癌样本中的38%、一些乳腺癌细胞系以及107份来自缩乳术的正常乳腺标本中的1.8%中，存在一段660碱基对的序列，该序列与小鼠乳腺肿瘤病毒的env基因有90 - 98%的同源性。在本报告中，我们通过逆转录聚合酶链反应研究了660碱基对序列或660碱基对内一个较小的256碱基对序列在乳腺肿瘤、正常乳腺组织及乳腺癌细胞系中是否表达。结果表明，在所研究的序列阳性乳腺癌肿瘤中，66%检测到了660碱基对序列的表达，而在阴性肿瘤或正常乳腺组织中均未检测到；并且在所有检测的env阳性乳腺癌细胞系中均检测到了256碱基对序列的表达。序列分析显示，所表达的660碱基对序列与小鼠乳腺肿瘤病毒的env基因有98%的同源性，仅有一小段46碱基对与内源性病毒序列同源。该序列的存在及表达与一组人类乳腺癌相关，在这组乳腺癌中可以考虑逆转录病毒病因的概念。
