Kaukonen K, Savolainen J, Viander M, Kotimaa M, Terho E O
Department of Clinical Allergology, University of Turku, Finland.
Clin Exp Allergy. 1993 Oct;23(10):851-6. doi: 10.1111/j.1365-2222.1993.tb00263.x.
Fifty patients with farmer's lung disease (FL), 20 asymptomatic exposed farmers, and 18 healthy controls were investigated for total IgG and total IgA, as well as subclass antibody levels against Aspergillus umbrosus, the most common fungus to which FL patients in Finland have been exposed. Enzyme-linked immunosorbent assay (ELISA) revealed significantly elevated levels of specific total IgG and IgA antibodies in the sera of patients during the acute phase of the disease. Both IgG1 and IgG2 subclass antibodies and both IgA1 and IgA2 subclass antibodies were significantly associated with the disease. The antibody levels decreased during the first 3 months, when mould exposure was avoided and when an oral 6-week steroid treatment was administered. During the next 12 month follow-up, the mean antibody levels continued to decrease, even though re-exposure might have occurred. The results (with sensitivity 94% and specificity 95%) imply that in addition to the measurement of A. umbrosus-specific IgG, determination of both A. umbrosus-specific IgG1 and IgA might also be useful in the serodiagnosis of acute stage of FL.
对50例农民肺疾病（FL）患者、20例无症状的暴露农民以及18名健康对照者进行了总IgG和总IgA以及针对荫蔽曲霉的亚类抗体水平检测，荫蔽曲霉是芬兰FL患者最常暴露的真菌。酶联免疫吸附测定（ELISA）显示，在疾病急性期患者血清中特异性总IgG和IgA抗体水平显著升高。IgG1和IgG2亚类抗体以及IgA1和IgA2亚类抗体均与该疾病显著相关。在避免接触霉菌并进行为期6周的口服类固醇治疗后的前3个月，抗体水平下降。在接下来的12个月随访中，尽管可能再次接触，但平均抗体水平持续下降。结果（敏感性为94%，特异性为95%）表明，除了检测荫蔽曲霉特异性IgG外，测定荫蔽曲霉特异性IgG1和IgA在FL急性期的血清诊断中可能也有用。
