Kaukonen K, Savolainen J, Viander M, Terho E O
Department of Clinical Allergology, University of Turku, Finland.
Clin Exp Allergy. 1993 Jan;23(1):21-7. doi: 10.1111/j.1365-2222.1993.tb02479.x.
Twenty-one different biotinylated lectins were used to recognize the carbohydrates of Aspergillus umbrosus, one of the most common microbes that patients with farmer's lung in Finland are exposed to. The glycoprotein fraction of A. umbrosus was bound especially well by Concanavalin A and consisted mainly of carbohydrates mannose and glucose. The carbohydrate fraction of A. umbrosus antigens were isolated from the crude extract of A. umbrosus with Con A-Sepharose affinity chromatography. Serum IgG antibodies to A. umbrosus mannoprotein fraction were determined in 57 patients with farmer's lung, 10 asymptomatic exposed farmers and 10 healthy controls by enzyme linked immunosorbent assay. Candida albicans and Saccharomyces cerevisiae mannan were used as controls. Patients had high levels, whereas asymptomatic exposed farmers showed moderate, and health controls low levels, of IgG antibodies to the A. umbrosus mannan/mannoprotein fraction. There were no significant differences in the mean antibody levels between the patients and controls against C. albicans or S. cerevisiae mannose fraction although in all groups more antibodies were detected against C. albicans than S. cerevisiae.
使用21种不同的生物素化凝集素来识别荫蔽曲霉的碳水化合物，荫蔽曲霉是芬兰农民肺患者接触的最常见微生物之一。刀豆球蛋白A与荫蔽曲霉的糖蛋白部分结合特别良好，其主要由碳水化合物甘露糖和葡萄糖组成。荫蔽曲霉抗原的碳水化合物部分通过伴刀豆球蛋白A - 琼脂糖亲和层析从荫蔽曲霉的粗提物中分离出来。通过酶联免疫吸附测定法测定了57例农民肺患者、10名无症状暴露农民和10名健康对照者中针对荫蔽曲霉甘露糖蛋白部分的血清IgG抗体。白色念珠菌和酿酒酵母甘露聚糖用作对照。患者针对荫蔽曲霉甘露聚糖/甘露糖蛋白部分的IgG抗体水平较高，而无症状暴露农民的水平中等，健康对照者的水平较低。患者和对照者针对白色念珠菌或酿酒酵母甘露糖部分的平均抗体水平没有显著差异，尽管在所有组中检测到的针对白色念珠菌的抗体比酿酒酵母更多。
