Nimnoi Pongrawee, Pongsilp Neelawan
Department of Microbiology, Faculty of Liberal Arts and Science, Kasetsart University, Nakhon Pathom 73140, Thailand.
Department of Microbiology, Faculty of Science, Silpakorn University, Nakhon Pathom 73000, Thailand.
Microorganisms. 2022 Feb 26;10(3):511. doi: 10.3390/microorganisms10030511.
Enterobacteria that commonly inhabit marine environments have a great impact on human health. In this study, enterobacteria isolated from seawater in the Upper Gulf of Thailand were identified and characterized. Seawater from nine sampling sites along the Upper Gulf of Thailand contained presumptive enterobacteria that ranged from 0.22 ± 0.44 to 17.00 ± 3.97 CFU/mL. The 101 strains belonged to seven species in which was the majority (47.5% of strains). The highest prevalence was resistant to ampicillin (76.2%) and ticarcillin (72.3%), respectively, whereas none was resistant to imipenem. Forty-five antibiotic resistance patterns were observed and 33.7% exhibited multidrug resistance, emphasizing the concern about public health. Three β-lactamase genes, including , , and , were detected at the frequencies of 47.5%, 21.8%, and 11.9%, respectively. Six virulence genes, including , , , , , and , were detected at the frequencies of 37.6%, 31.7%, 19.8%, 16.8%, 12.9%, and 5.9%, respectively. The condition of 4% NaCl downregulated the expression of the and genes. The 67.3% and 63.4% of strains synthesized silver nanoparticles ranging between 3.04 ± 0.64 and 20.64 ± 0.95 μg/mL and gold nanoparticles ranging between 7.77 ± 0.45 and 57.57 ± 8.00 μg/mL, respectively.
通常栖息于海洋环境中的肠杆菌对人类健康有重大影响。在本研究中，对从泰国湾北部海水中分离出的肠杆菌进行了鉴定和表征。泰国湾北部九个采样点的海水中，推定的肠杆菌含量在0.22±0.44至17.00±3.97 CFU/mL之间。101株菌株属于7个物种，其中 占多数（占菌株的47.5%）。最高的耐药率分别是对氨苄西林（76.2%）和替卡西林（72.3%），而对亚胺培南均无耐药性。观察到45种抗生素耐药模式，33.7%表现出多重耐药性，这凸显了对公共卫生的担忧。检测到三种β-内酰胺酶基因，包括 、 和 ，频率分别为47.5%、21.8%和11.9%。检测到六种毒力基因，包括 、 、 、 、 和 ，频率分别为37.6%、31.7%、19.8%、16.8%、12.9%和5.9%。4% NaCl的条件下调了 和 基因的表达。分别有67.3%和63.4%的菌株合成了银纳米颗粒，范围在3.04±0.64至20.64±0.95 μg/mL之间，以及金纳米颗粒，范围在7.77±0.45至57.57±8.00 μg/mL之间。
