多核苷酸。二十六。源自间型霉素和月桂霉素的多核苷酸与环核苷寡核苷酸的复合物形成
Polynucleotides. XXVI. Complex formation of polynucleotides derived from formycin and laurusin with cyclonucleoside oligonucleotides.
作者信息
Ikehara M, Tezuka T
出版信息
Nucleic Acids Res. 1974 Jul;1(7):907-17. doi: 10.1093/nar/1.7.907.
Poly(formycin phosphate) and poly(laurusin phosphate) were synthesized by polymerizing formycin and laurusin 5'-diphosphate by means of E. coli polynucleotide phosphorylase. The complex formation of these polynucleotides with cyclonucleoside polynucleotides were investigated. While poly(formycin phosphate) did not form the complex with an octanucleotide of 6,2'-anhydro-6-oxy-1-beta-D-arabinofuranosyluracil, poly(laurusin phosphate) did form a 1: 1 complex with octanucleotide of 8,2'-anhydro-8-mercapto-9-beta-D-arabinofuranosyladenine in the presence of 0.15M Na ion at neutrality and 3(o). CD spectrum of this complex showed a couple of a trough at 286 nm and a peak at 262 nm. This fact suggests that the complex has a left-handed helical conformation, which is opposite to the natural double helical polynucleotides. The cause of this phenomenon was discussed in connection with the complex of cyclonucleoside oligonucleotides.
通过大肠杆菌多核苷酸磷酸化酶使甲酰霉素和月桂霉素5'-二磷酸聚合，合成了聚（甲酰霉素磷酸）和聚（月桂霉素磷酸）。研究了这些多核苷酸与环核苷多核苷酸的复合物形成情况。虽然聚（甲酰霉素磷酸）不与6,2'-脱水-6-氧代-1-β-D-阿拉伯呋喃糖基尿嘧啶的八核苷酸形成复合物，但聚（月桂霉素磷酸）在中性条件下0.15M钠离子存在及3℃时，确实与8,2'-脱水-8-巯基-9-β-D-阿拉伯呋喃糖基腺嘌呤的八核苷酸形成了1:1复合物。该复合物的圆二色光谱在286nm处有一个谷，在262nm处有一个峰。这一事实表明该复合物具有左手螺旋构象，这与天然的双螺旋多核苷酸相反。结合环核苷寡核苷酸的复合物讨论了这种现象的原因。
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