Cassio D, Mathien Y
Nucleic Acids Res. 1974 May;1(5):719-25. doi: 10.1093/nar/1.5.719.
In cells of E.coli K12 grown exponentially in minimal medium, tRNA(met), tRNA(leu) and tRNA(ile) are aminoacylated at 100%, 80% and 64%, respectively. On addition of L-methioninyl adenylate to the growth medium, one observes a specific deacylation of tRNA(met). When more than 35% of tRNA(met) is deacylated, growth rate is reduced and becomes proportional to the amount of methionyl-tRNA formed.
在以基本培养基指数生长的大肠杆菌K12细胞中,tRNA(met)、tRNA(leu)和tRNA(ile)的氨酰化率分别为100%、80%和64%。向生长培养基中添加L-甲硫氨酰腺苷酸后,可观察到tRNA(met)发生特异性去氨酰化。当超过35%的tRNA(met)去氨酰化时,生长速率降低,并与形成的甲硫氨酰-tRNA量成正比。